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primary topic: Label-free plasma proteomics for the identification of the putative biomarkers of oral squamous cell carcinoma

Label-free plasma proteomics for the identification of the putative biomarkers of oral squamous cell carcinoma
type: AcademicArticle
type: Article
abstract: Mass spectrometry-based label-free proteomics is becoming the analytical tool of interest to identify and quantitate the biomarkers for cancer. The oral squamous cell carcinoma (OSCC) which is one of the leading cancers worldwide, lacks biomarkers for the early prognosis and diagnosis. The present study profiled plasma proteome of the Indian OSCC human patients using a label-free mass spectrometry proteomics approach. The study first time utilized the three most widely used data analysis software MaxQuant (MQ), Proteome discoverer (PD), and Trans proteomic pipeline (TPP) together for label-free quantitation of the proteins. The study identified 16 proteins which can be used as a signature protein panel for OSCC. The pathway analysis showed predominant involvement of the immune system, hemostasis as the major pathways that are indicative of the disease progression. The network analysis showed maximum interaction for the Fibronectin and C-reactive protein. The study demonstrates that plasma proteins contain signatures that can be used as putative biomarkers for OSCC. Based on the label-free quantitation and the mechanistic analysis C-reactive protein, Carbonic anhydrase-1, and Fibronectin are identified as putative biomarkers of OSCC. Once these findings are validated in the large cohorts these protein signatures can be used as a biomarker for OSCC. SIGNIFICANCE: The oral squamous cell carcinoma (OSCC) is the eighteenth most prevalent malignancy in the world and ranks second in India. There are no biomarkers that could be indicative of the diseased state. Various studies have been carried out on saliva and tumors of OSCC patients in India, but none of the studies have profiled the plasma. We utilized the label-free approach for the first time on the Indian population in generating the panel of plasma proteins which could be indicative of the diseased state. The study identified Carbonic anhydrase 1, C-reactive protein, and Fibronectin proteins as putative biomarkers f…
date: 2022-05-15
volume: 259
is part of: Journal of proteomics
status: peerReviewed
status: published
author list: authors
type: EPrint
type: ArticleEPrint
is part of: repository
subject: QR Microbiology

Elsevier Science
type: Organization

Journal of proteomics
type: Collection
issn: 18767737

Rashmi Kumar
type: Person
given name: Rashmi
family name: Kumar

Sudhir Singh
type: Person
given name: Sudhir
family name: Singh

Raghwendra Pratap Singh
type: Person
given name: Raghwendra Pratap
family name: Singh

Panga Jaipal Reddy
type: Person
given name: Panga Jaipal
family name: Reddy

Shashyendra Singh Gautam
type: Person
given name: Shashyendra Singh
family name: Gautam

Sheelendra Pratap Singh
type: Person
given name: Sheelendra Pratap
family name: Singh

Divya Mehrotra
type: Person
given name: Divya
family name: Mehrotra

Sameer Gupta
type: Person
given name: Sameer
family name: Gupta

Kajal Karsauliya
type: Person
given name: Kajal
family name: Karsauliya

Ashish Kumar Sonker
type: Person
given name: Ashish Kumar
family name: Sonker

HTML Summary of #2974 Label-free plasma proteomics for the identification of the putative biomarkers of oral squamous cell carcinoma
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primary topic: Label-free plasma proteomics for the identification of the putative biomarkers of oral squamous cell carcinoma

QR Microbiology
type: Concept