<> "The repository administrator has not yet configured an RDF license."^^ . <> . . . "Plasmodium falciparum Kelch13 and its artemisinin-resistant mutants assemble as hexamers in solution: a SAXS data-driven modelling study"^^ . "The artemisinin-resistant mutations in Plasmodium falciparum (PfKelch13) identified worldwide are mostly confined to the Broad-complex, tramtrack and bric-à-brac/poxvirus and zinc-finger (BTB/POZ) and Kelch-repeat propeller (KRP) domains. To date, only two crystal structures of the BTB/POZ-KRP domains as tight dimers are available, which limits structure-based predictions and interpretation of its role(s) in inducing clinical artemisinin resistance. Our solution Small-Angle X-ray Scattering (SAXS) data analysis and shape restoration brought forth that: (a) PfKelch13 forms a stable hexamer in P6 symmetry, (b) interactions of the N-termini drive the hexameric assembly, and (c) the six KRP domains project independently in space, forming a cauldron-like architecture. We further deduce that the artemisinin-sensitive mutant A578S is packed like the wild-type protein, however, hexameric assemblies of the predominant artemisinin-resistant mutants R539T and C580Y displayed detectable differences in the spatial positioning of their BTB/POZ-KRP domains. Lastly, mapping of mutations known to enable artemisinin resistance suggested evolutionary pressure in the selection for mutations in the BTB/POZ-KRP domains. These mutations appear non-detrimental to the hexameric assembly of proteins, and yet somehow alter the flux of downstream events essential for the susceptibility to artemisinin."^^ . "2022-01-28" . . "Wiley"^^ . . . "The FEBS journal"^^ . . . "17424658" . . . . . . . . . . . . . . . . . . . . . . "Anwesha "^^ . "Mukhopadhyay"^^ . "Anwesha Mukhopadhyay"^^ . . "Fnu"^^ . "Ashish"^^ . "Fnu Ashish"^^ . . "Nainy "^^ . "Goel"^^ . "Nainy Goel"^^ . . "Nidhi "^^ . "Kalidas"^^ . "Nidhi Kalidas"^^ . . "Kanika "^^ . "Dhiman"^^ . "Kanika Dhiman"^^ . . "Souvik"^^ . "Bhattacharjee"^^ . "Souvik Bhattacharjee"^^ . . . . . "HTML Summary of #2976 \n\nPlasmodium falciparum Kelch13 and its artemisinin-resistant mutants assemble as hexamers in solution: a SAXS data-driven modelling study\n\n" . "text/html" . . . "QR Microbiology"@en . .