@article{open2979,
           month = {April},
           title = {A comparative study of in-vitro and in-silico anti-candidal activity and GC-MS profiles of snow mountain garlic vs. normal garlic},
          author = {Bhupinder  Kaur  and Nitish  Kumar and Sonam  Chawla and Deepika  Sharma and Suresh  Korpole  and Rajni  Sharma and Manoj K  Patel and Kanwaljit  Chopra and Om Prakash  Chaurasia and Shweta  Saxena},
       publisher = {Wiley},
            year = {2022},
            note = {The copyright of this article belongs to Wiley},
         journal = {Journal of applied microbiology},
        keywords = {Candida-cidal activity; anti-fungal drug resistance; exo-1,3 beta glucan synthase; phytoactive compounds; snow mountain garlic},
             url = {http://crdd.osdd.net/open/2979/},
        abstract = {Aim: The study aimed to profile the volatile phytocomposition of snow mountain garlic (SMG) compared to normal garlic and investigate the anti-Candida efficacy against clinically relevant multi-drug resistant isolates of Candida species.

Methods and results: Herein, SMG has shown significantly superior fungicidal power at 2x-MIC dose against C. albicans and C. glabrata in killing kinetic evaluation unlike the fungistatic effect of normal garlic. GC-MS headspace-based profiling of SMG showed 5 unique volatile compounds and a 5-fold higher content of saponins than normal garlic. In an in-silico analysis, cholesta-4,6-dien-3-ol,(3-beta) was uniquely identified in SMG as a potential inhibitor with high binding affinity to the active site of exo-1,3-betaglucan synthase, an established anti-candida drug target crucial for the biofilm matrix formation, thus suggesting a plausible anti-Candida mechanism.

Conclusion: The in-vitro and in-silico studies have demonstrated the Candida-cidal and anti-biofilm activities of SMG, distinguishing it from the Candida-static efficacy of normal garlic.}
}