title: A comparative study of in-vitro and in-silico anti-candidal activity and GC-MS profiles of snow mountain garlic vs. normal garlic
creator: Kaur , Bhupinder 
creator: Kumar, Nitish 
creator: Chawla, Sonam 
creator: Sharma, Deepika 
creator: Korpole , Suresh 
creator: Sharma, Rajni 
creator: Patel, Manoj K 
creator: Chopra, Kanwaljit 
creator: Chaurasia, Om Prakash 
creator: Saxena, Shweta 
subject: QR Microbiology
description: Aim: The study aimed to profile the volatile phytocomposition of snow mountain garlic (SMG) compared to normal garlic and investigate the anti-Candida efficacy against clinically relevant multi-drug resistant isolates of Candida species.    Methods and results: Herein, SMG has shown significantly superior fungicidal power at 2x-MIC dose against C. albicans and C. glabrata in killing kinetic evaluation unlike the fungistatic effect of normal garlic. GC-MS headspace-based profiling of SMG showed 5 unique volatile compounds and a 5-fold higher content of saponins than normal garlic. In an in-silico analysis, cholesta-4,6-dien-3-ol,(3-beta) was uniquely identified in SMG as a potential inhibitor with high binding affinity to the active site of exo-1,3-betaglucan synthase, an established anti-candida drug target crucial for the biofilm matrix formation, thus suggesting a plausible anti-Candida mechanism.    Conclusion: The in-vitro and in-silico studies have demonstrated the Candida-cidal and anti-biofilm activities of SMG, distinguishing it from the Candida-static efficacy of normal garlic.
publisher: Wiley
date: 2022-04-07
type: Article
type: PeerReviewed
relation: https://sfamjournals.onlinelibrary.wiley.com/doi/10.1111/jam.15537
identifier:   Kaur , Bhupinder  and Kumar, Nitish  and Chawla, Sonam  and Sharma, Deepika  and Korpole , Suresh  and Sharma, Rajni  and Patel, Manoj K  and Chopra, Kanwaljit  and Chaurasia, Om Prakash  and Saxena, Shweta   (2022) A comparative study of in-vitro and in-silico anti-candidal activity and GC-MS profiles of snow mountain garlic vs. normal garlic.  Journal of applied microbiology.   ISSN 1364-5072     
relation: http://crdd.osdd.net/open/2979/