%0 Journal Article
%@ 1364-5072
%A Kaur , Bhupinder 
%A Kumar, Nitish 
%A Chawla, Sonam 
%A Sharma, Deepika 
%A Korpole , Suresh 
%A Sharma, Rajni 
%A Patel, Manoj K 
%A Chopra, Kanwaljit 
%A Chaurasia, Om Prakash 
%A Saxena, Shweta 
%D 2022
%F open:2979
%I Wiley
%J Journal of applied microbiology
%K Candida-cidal activity; anti-fungal drug resistance; exo-1,3 beta glucan synthase; phytoactive compounds; snow mountain garlic
%T A comparative study of in-vitro and in-silico anti-candidal activity and GC-MS profiles of snow mountain garlic vs. normal garlic
%U http://crdd.osdd.net/open/2979/
%X Aim: The study aimed to profile the volatile phytocomposition of snow mountain garlic (SMG) compared to normal garlic and investigate the anti-Candida efficacy against clinically relevant multi-drug resistant isolates of Candida species.    Methods and results: Herein, SMG has shown significantly superior fungicidal power at 2x-MIC dose against C. albicans and C. glabrata in killing kinetic evaluation unlike the fungistatic effect of normal garlic. GC-MS headspace-based profiling of SMG showed 5 unique volatile compounds and a 5-fold higher content of saponins than normal garlic. In an in-silico analysis, cholesta-4,6-dien-3-ol,(3-beta) was uniquely identified in SMG as a potential inhibitor with high binding affinity to the active site of exo-1,3-betaglucan synthase, an established anti-candida drug target crucial for the biofilm matrix formation, thus suggesting a plausible anti-Candida mechanism.    Conclusion: The in-vitro and in-silico studies have demonstrated the Candida-cidal and anti-biofilm activities of SMG, distinguishing it from the Candida-static efficacy of normal garlic.
%Z The copyright of this article belongs to Wiley