TY  - JOUR
N1  - The copyright of this article belongs to Wiley
ID  - open2979
UR  - https://sfamjournals.onlinelibrary.wiley.com/doi/10.1111/jam.15537
A1  - Kaur , Bhupinder 
A1  - Kumar, Nitish 
A1  - Chawla, Sonam 
A1  - Sharma, Deepika 
A1  - Korpole , Suresh 
A1  - Sharma, Rajni 
A1  - Patel, Manoj K 
A1  - Chopra, Kanwaljit 
A1  - Chaurasia, Om Prakash 
A1  - Saxena, Shweta 
Y1  - 2022/04/07/
N2  - Aim: The study aimed to profile the volatile phytocomposition of snow mountain garlic (SMG) compared to normal garlic and investigate the anti-Candida efficacy against clinically relevant multi-drug resistant isolates of Candida species.

Methods and results: Herein, SMG has shown significantly superior fungicidal power at 2x-MIC dose against C. albicans and C. glabrata in killing kinetic evaluation unlike the fungistatic effect of normal garlic. GC-MS headspace-based profiling of SMG showed 5 unique volatile compounds and a 5-fold higher content of saponins than normal garlic. In an in-silico analysis, cholesta-4,6-dien-3-ol,(3-beta) was uniquely identified in SMG as a potential inhibitor with high binding affinity to the active site of exo-1,3-betaglucan synthase, an established anti-candida drug target crucial for the biofilm matrix formation, thus suggesting a plausible anti-Candida mechanism.

Conclusion: The in-vitro and in-silico studies have demonstrated the Candida-cidal and anti-biofilm activities of SMG, distinguishing it from the Candida-static efficacy of normal garlic.
PB  - Wiley
JF  - Journal of applied microbiology
KW  - Candida-cidal activity; anti-fungal drug resistance; exo-1
KW  - 3 beta glucan synthase; phytoactive compounds; snow mountain garlic
SN  - 1364-5072
TI  - A comparative study of in-vitro and in-silico anti-candidal activity and GC-MS profiles of snow mountain garlic vs. normal garlic
ER  -