%A Bhupinder  Kaur 
%A Nitish  Kumar
%A Sonam  Chawla
%A Deepika  Sharma
%A Suresh  Korpole 
%A Rajni  Sharma
%A Manoj K  Patel
%A Kanwaljit  Chopra
%A Om Prakash  Chaurasia
%A Shweta  Saxena
%O The copyright of this article belongs to Wiley
%J Journal of applied microbiology
%T A comparative study of in-vitro and in-silico anti-candidal activity and GC-MS profiles of snow mountain garlic vs. normal garlic
%X Aim: The study aimed to profile the volatile phytocomposition of snow mountain garlic (SMG) compared to normal garlic and investigate the anti-Candida efficacy against clinically relevant multi-drug resistant isolates of Candida species.

Methods and results: Herein, SMG has shown significantly superior fungicidal power at 2x-MIC dose against C. albicans and C. glabrata in killing kinetic evaluation unlike the fungistatic effect of normal garlic. GC-MS headspace-based profiling of SMG showed 5 unique volatile compounds and a 5-fold higher content of saponins than normal garlic. In an in-silico analysis, cholesta-4,6-dien-3-ol,(3-beta) was uniquely identified in SMG as a potential inhibitor with high binding affinity to the active site of exo-1,3-betaglucan synthase, an established anti-candida drug target crucial for the biofilm matrix formation, thus suggesting a plausible anti-Candida mechanism.

Conclusion: The in-vitro and in-silico studies have demonstrated the Candida-cidal and anti-biofilm activities of SMG, distinguishing it from the Candida-static efficacy of normal garlic.
%K Candida-cidal activity; anti-fungal drug resistance; exo-1,3 beta glucan synthase; phytoactive compounds; snow mountain garlic
%D 2022
%I Wiley
%L open2979