creators_name: Kaur , Bhupinder 
creators_name: Kumar, Nitish 
creators_name: Chawla, Sonam 
creators_name: Sharma, Deepika 
creators_name: Korpole , Suresh 
creators_name: Sharma, Rajni 
creators_name: Patel, Manoj K 
creators_name: Chopra, Kanwaljit 
creators_name: Chaurasia, Om Prakash 
creators_name: Saxena, Shweta 
type: article
datestamp: 2022-07-22 04:47:32
lastmod: 2022-07-22 04:47:32
metadata_visibility: show
title: A comparative study of in-vitro and in-silico anti-candidal activity and GC-MS profiles of snow mountain garlic vs. normal garlic
ispublished: pub
subjects: QR
keywords: Candida-cidal activity; anti-fungal drug resistance; exo-1,3 beta glucan synthase; phytoactive compounds; snow mountain garlic
note: The copyright of this article belongs to Wiley
abstract: Aim: The study aimed to profile the volatile phytocomposition of snow mountain garlic (SMG) compared to normal garlic and investigate the anti-Candida efficacy against clinically relevant multi-drug resistant isolates of Candida species.

Methods and results: Herein, SMG has shown significantly superior fungicidal power at 2x-MIC dose against C. albicans and C. glabrata in killing kinetic evaluation unlike the fungistatic effect of normal garlic. GC-MS headspace-based profiling of SMG showed 5 unique volatile compounds and a 5-fold higher content of saponins than normal garlic. In an in-silico analysis, cholesta-4,6-dien-3-ol,(3-beta) was uniquely identified in SMG as a potential inhibitor with high binding affinity to the active site of exo-1,3-betaglucan synthase, an established anti-candida drug target crucial for the biofilm matrix formation, thus suggesting a plausible anti-Candida mechanism.

Conclusion: The in-vitro and in-silico studies have demonstrated the Candida-cidal and anti-biofilm activities of SMG, distinguishing it from the Candida-static efficacy of normal garlic.
date: 2022-04-07
date_type: published
publication: Journal of applied microbiology
publisher: Wiley
refereed: TRUE
issn: 1364-5072
official_url: https://sfamjournals.onlinelibrary.wiley.com/doi/10.1111/jam.15537
citation:   Kaur , Bhupinder  and Kumar, Nitish  and Chawla, Sonam  and Sharma, Deepika  and Korpole , Suresh  and Sharma, Rajni  and Patel, Manoj K  and Chopra, Kanwaljit  and Chaurasia, Om Prakash  and Saxena, Shweta   (2022) A comparative study of in-vitro and in-silico anti-candidal activity and GC-MS profiles of snow mountain garlic vs. normal garlic.  Journal of applied microbiology.   ISSN 1364-5072