title: Host glyceraldehyde-3-phosphate dehydrogenase-mediated iron acquisition is hijacked by intraphagosomal Mycobacterium tuberculosis
creator: Patidar , Anil 
creator: Malhotra, Himanshu 
creator: Chaudhary, Surbhi 
creator: Kumar, Manoj 
creator: Dilawari, Rahul 
creator: Chaubey, Gaurav Kumar 
creator: Dhiman, Asmita 
creator: Modanwal , Radheshyam 
creator: Talukdar, Sharmila 
creator: Raje , Chaaya Iyengar 
creator: Raje, Manoj 
subject: QR Microbiology
description: Availability of iron is a key factor in the survival and multiplication of Mycobacterium tuberculosis (M.tb) within host macrophage phagosomes. Despite host cell iron regulatory machineries attempts to deny supply of this essential micronutrient, intraphagosomal M.tb continues to access extracellular iron. In the current study, we report that intracellular M.tb exploits mammalian secreted Glyceraldehyde 3-phosphate dehydrogenase (sGAPDH) for the delivery of host iron carrier proteins lactoferrin (Lf) and transferrin (Tf). Studying the trafficking of iron carriers in infected cells we observed that sGAPDH along with the iron carrier proteins are preferentially internalized into infected cells and trafficked to M.tb containing phagosomes where they are internalized by resident mycobacteria resulting in iron delivery. Collectively our findings provide a new mechanism of iron acquisition by M.tb involving the hijack of host sGAPDH. This may contribute to its successful pathogenesis and provide an option for targeted therapeutic intervention.
publisher: Springer 
date: 2022
type: Article
type: PeerReviewed
relation: https://link.springer.com/article/10.1007/s00018-021-04110-3
identifier:   Patidar , Anil  and Malhotra, Himanshu  and Chaudhary, Surbhi  and Kumar, Manoj  and Dilawari, Rahul  and Chaubey, Gaurav Kumar  and Dhiman, Asmita  and Modanwal , Radheshyam  and Talukdar, Sharmila  and Raje , Chaaya Iyengar  and Raje, Manoj   (2022) Host glyceraldehyde-3-phosphate dehydrogenase-mediated iron acquisition is hijacked by intraphagosomal Mycobacterium tuberculosis.  Cellular and Molecular Life Sciences , 79 (1).       
relation: http://crdd.osdd.net/open/2989/