creators_name: Patidar , Anil 
creators_name: Malhotra, Himanshu 
creators_name: Chaudhary, Surbhi 
creators_name: Kumar, Manoj 
creators_name: Dilawari, Rahul 
creators_name: Chaubey, Gaurav Kumar 
creators_name: Dhiman, Asmita 
creators_name: Modanwal , Radheshyam 
creators_name: Talukdar, Sharmila 
creators_name: Raje , Chaaya Iyengar 
creators_name: Raje, Manoj 
type: article
datestamp: 2022-07-25 05:39:36
lastmod: 2022-07-25 05:39:36
metadata_visibility: show
title: Host glyceraldehyde-3-phosphate dehydrogenase-mediated iron acquisition is hijacked by intraphagosomal Mycobacterium tuberculosis
ispublished: pub
subjects: QR
keywords: GAPDH; Iron; Lactoferrin; Lipoarabinomannan (LAM); M.tb; Targeted delivery; Transferrin
note: The copyright of this article belongs to Springer 
abstract: Availability of iron is a key factor in the survival and multiplication of Mycobacterium tuberculosis (M.tb) within host macrophage phagosomes. Despite host cell iron regulatory machineries attempts to deny supply of this essential micronutrient, intraphagosomal M.tb continues to access extracellular iron. In the current study, we report that intracellular M.tb exploits mammalian secreted Glyceraldehyde 3-phosphate dehydrogenase (sGAPDH) for the delivery of host iron carrier proteins lactoferrin (Lf) and transferrin (Tf). Studying the trafficking of iron carriers in infected cells we observed that sGAPDH along with the iron carrier proteins are preferentially internalized into infected cells and trafficked to M.tb containing phagosomes where they are internalized by resident mycobacteria resulting in iron delivery. Collectively our findings provide a new mechanism of iron acquisition by M.tb involving the hijack of host sGAPDH. This may contribute to its successful pathogenesis and provide an option for targeted therapeutic intervention.
date: 2022
date_type: published
publication: Cellular and Molecular Life Sciences 
volume: 79
number: 1
publisher: Springer 
refereed: TRUE
official_url: https://link.springer.com/article/10.1007/s00018-021-04110-3
citation:   Patidar , Anil  and Malhotra, Himanshu  and Chaudhary, Surbhi  and Kumar, Manoj  and Dilawari, Rahul  and Chaubey, Gaurav Kumar  and Dhiman, Asmita  and Modanwal , Radheshyam  and Talukdar, Sharmila  and Raje , Chaaya Iyengar  and Raje, Manoj   (2022) Host glyceraldehyde-3-phosphate dehydrogenase-mediated iron acquisition is hijacked by intraphagosomal Mycobacterium tuberculosis.  Cellular and Molecular Life Sciences , 79 (1).