@article{open2999,
          volume = {26},
           month = {May},
          author = {Nitish Kumar Verma and Sachin Kumar and G S Prasad and Mani Shankar Bhattacharyya},
            note = {The copyright  of this article belongs to Elsevier },
           title = {Biocatalytic deracemization of racemic naphthyl alcohols by using a novel yeast isolate Rhodotorula kratochvilovae (MTCC 13029)},
       publisher = {Elsevier},
         journal = {Sustainable Chemistry and Pharmacy},
            year = {2022},
        keywords = {Stereoinversion; Rhodotorula kratochvilovae; Deracemization; Reductase; Oxidase},
             url = {http://crdd.osdd.net/open/2999/},
        abstract = {Enantiopure alcohols are key intermediates for the synthesis of a plethora of pharmaceuticals, agrochemicals and fine chemicals. Present study reports the deracemization of racemic naphthyl ethanols into their enantiopure S-forms by CHF-15P (Rhodotorula kratochvilovae, MTCC 13029) whole cells, isolated from the high altitude of the Himalayan mountain range. Initially, the biocatalyst was able to deracemize rac-1-(6-methoxy-2-naphthyl)ethanol to S-1-(6-methoxy-2-naphthyl)ethanol with 86.3\% yield and 82.6\% eeS. The studies on the effect of physicochemical parameters showed 92.3\% yield with eeS{\ensuremath{>}}99\% of product in a medium containing 2 mM substrate concentration and 2\% glucose at 30 ?C temperature, pH 7.0 (0.2 M sodium phosphate buffer), with 100 mg/mL cell concentration. In addition, the deracemization of rac-1-(6-methyl-2-naphthyl)ethanol and rac-1-(2-naphthyl)ethanol was achieved within 12 h at 30 ?C with 96.4 and 98.8\% of yield (98.2\% and {\ensuremath{>}}99\% eeS), respectively. The process of deracemization occurs via the formation of a ketone intermediate. The appearance of ketone has been demonstrated by high performance liquid chromatography (HPLC) and confocal laser scanning microscopy. However, rac-1-(1-naphthyl)ethanol and rac-1-(4-fluoro-1-naphthyl)ethanol were not deracemized under the same condition. In gram scale preparation, 92.8\% yield with eeS{\ensuremath{>}}99\% was obtained.}
}