creators_name: Mukherjee, K
creators_name: Parashuraman, S
creators_name: Raje, Manoj
creators_name: Mukhopadhyay, A
type: article
datestamp: 2012-01-04 14:57:12
lastmod: 2015-01-07 05:54:28
metadata_visibility: show
title: SopE acts as an Rab5-specific nucleotide exchange factor and recruits non-prenylated Rab5 on Salmonella-containing phagosomes to promote fusion with early endosomes.
ispublished: pub
subjects: QD
full_text_status: restricted
note: Copyright of this article belongs to ASBMB
abstract: Rab-GTPase regulates the fusion between two specific vesicles. It is well documented that, for their biological function, Rab proteins need to be prenylated for attachment to the vesicle membrane. In contrast, we showed in the present investigation that SopE, a type III secretory protein of Salmonella, translocates onto Salmonella-containing phagosomes (LSP) and mediates the recruitment of non-prenylated Rab5 (Rab5:DeltaC4) on LSP in GTP form. Simultaneously, SopE present in infected cell cytosol acts as an Rab5-specific exchange factor and converts the inactive Rab-GDP to the GTP form. The non-prenylated Rab5 subsequently promoted efficient fusion of LSP with early endosomes. This is the first demonstration that a prenylation-deficient Rab protein retains biological activity and can promote vesicle fusion, if it is recruited on the membrane by some other method.
date: 2001-06-29
date_type: published
publication: The Journal of biological chemistry
volume: 276
number: 26
publisher: ASBMB
pagerange: 23607-15
refereed: TRUE
issn: 0021-9258
official_url: http://www.jbc.org/content/276/26/23607.full.pdf+html
related_url_url: http://www.jbc.org/content/276/26/23607.full.pdf+html
related_url_type: pub
citation:   Mukherjee, K and Parashuraman, S and Raje, Manoj and Mukhopadhyay, A  (2001) SopE acts as an Rab5-specific nucleotide exchange factor and recruits non-prenylated Rab5 on Salmonella-containing phagosomes to promote fusion with early endosomes.  The Journal of biological chemistry, 276 (26).  pp. 23607-15.  ISSN 0021-9258     
document_url: http://crdd.osdd.net/open/300/1/raje2001.pdf