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primary topic: K-edge anomalous SAXS for protein solution structure modeling

K-edge anomalous SAXS for protein solution structure modeling
type: AcademicArticle
type: Article
abstract: K-edge anomalous SAXS intensity was measured from a small, dimeric, partly unstructured protein segment of myosin X by using cupric ions bound to its C-terminal polyhistidine tags. Energy-dependent anomalous SAXS can provide key location-specific information about metal-labeled protein structures in solution that cannot be obtained from routine SAXS analysis. However, anomalous SAXS is seldom used for protein research due to practical difficulties, such as a lack of generic multivalent metal-binding tags and the challenges of measuring weak anomalous signal at the metal absorption edge. This pilot feasibility study suggests that weak K-edge anomalous SAXS signal can be obtained from transition metals bound to terminally located histidine tags of small proteins. The measured anomalous signal can provide information about the distribution of all metal-protein distances in the complex. Such an anomalous SAXS signal can assist in the modeling and validation of structured or unstructured proteins in solution and may potentially become a new addition to the repertoire of techniques in integrative structural biology.
date: 2022-02-01
volume: 78
is part of: Acta crystallographica. Section D, Structural biology
status: peerReviewed
status: published
author list: authors
type: EPrint
type: ArticleEPrint
is part of: repository
subject: QR Microbiology

International Union of Crystallography
type: Organization

Acta crystallographica. Section D, Structural biology
type: Collection
issn: 20597983

Lucky Singh
type: Person
given name: Lucky
family name: Singh

Barnali Chaudhuri
type: Person
given name: Barnali
family name: Chaudhuri

Kento Yonezawa
type: Person
given name: Kento
family name: Yonezawa

Nobutaka Shimizu
type: Person
given name: Nobutaka
family name: Shimizu

Karman Virk
type: Person
given name: Karman
family name: Virk

Komal Choukate
type: Person
given name: Komal
family name: Choukate

HTML Summary of #3005 K-edge anomalous SAXS for protein solution structure modeling
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primary topic: K-edge anomalous SAXS for protein solution structure modeling

QR Microbiology
type: Concept