creators_name: Kakkar, Saloni
creators_name: Chauhan, Sakshi
creators_name: Bala, Rajni
creators_name: Bharti, Bala
creators_name: Kumar, Virendra
creators_name: Rohit, Manojkumar
creators_name: Bhalla, Vijayender
type: article
datestamp: 2022-12-15 05:29:33
lastmod: 2022-12-15 05:29:33
metadata_visibility: show
title: Site-directed dual bioprobes inducing single-step nano-sandwich assay for the detection of cardiac troponin I
ispublished: pub
subjects: QR
keywords:  Aptamer; Gold nanoparticles; Nano-sandwich assay; Peptide; Site-directed nanobioprobes; Troponin I.
note: Copyright of this article belongs to Springer 
abstract: Bioreceptor functionalized metallic nano-colloids have been identified as effective nanobioprobes to realize the detection of an analyte based on a common phenomenon of salt-induced aggregation. In marked contrast to this, we describe a nano-sandwich assay integrating the novel match-pair of aptamer and peptide functionalized gold nanoparticles. The site-directed biomolecular interaction of high affinity aptamer and peptide bioreceptors directed towards distinct sites of cardiac biomarker troponin I; this was found to form a nano-sandwich assay in a peculiar manner. The gold nanoconjugates interact with specific and distant regions of troponin I to result in collision of probes upon target identification. In the presence of TnI, both nanobioprobes bind at their respective sites forming a nano-sandwich pair providing a visual color change from red to blue. Thus, the presence of target TnI itself causes instant agglomeration in just a single-step without addition of any external aggregator. The assay imparts 100% specificity and 90% sensitivity in a dynamic concentration range of 0.1-500 ng/mL troponin I with detection limit as low as 0.084 ng/mL. The applicability of the assay has been validated in clinical samples of acute myocardial infarction patients thus establishing a promising point-of-care detection of TnI.
date: 2022-10
date_type: published
publication: MICROCHIMICA ACTA
volume: 189
number: 10
publisher: Springer 
refereed: TRUE
official_url: https://link.springer.com/article/10.1007/s00604-022-05461-9
citation:   Kakkar, Saloni and Chauhan, Sakshi and Bala, Rajni and Bharti, Bala and Kumar, Virendra and Rohit, Manojkumar and Bhalla, Vijayender  (2022) Site-directed dual bioprobes inducing single-step nano-sandwich assay for the detection of cardiac troponin I.  MICROCHIMICA ACTA, 189 (10).