creators_name: Garg, Mansi
creators_name: Sharma, Deepak
creators_name: Kumar, Rajesh
type: article
datestamp: 2022-12-15 08:05:04
lastmod: 2022-12-15 08:05:04
metadata_visibility: show
title: Analysis of the effect of 1-Allyl-3-Methylimidazolium chloride on thermodynamic stability, folding kinetics, and motional dynamics of horse cytochrome c
ispublished: pub
subjects: QR
keywords: 1-allyl-3-methylimidazolium chloride; Cytochrome c; Folding kinetics; Motional dynamics; Thermodynamic stability
note: The copyright of this article belongs to Elsevier 
abstract: 1-allyl-3-methylimidazolium chloride (AMIMCl) acts as a potential green solvent for proteins. The present work provides a possible pathway by which the structural, kinetic, thermodynamic, and folding properties of horse cytochrome c (cyt c) are affected in green aqueous-AMIMCl systems. Analysis of the effect of AMIMCl on thermodynamic stability, refolding/unfolding kinetics, and motional dynamics of cyt c provided important information, (i) AMIMCl decreases the thermodynamic stability of reduced cyt c and also strengthens the guanidinium chloride (GdmCl)-mediated decrease in thermodynamic stability of protein, (ii) AMIMCl reduces the thermal-fluctuation of Met80-containing omega-loop of natively-folded compact state of carbonmonoxycytochrome c (MCO-state) due to polyfunctional interactions between the AMIM+ and different groups of protein, (iii) AMIMCl shifts the kinetic chevron plot, ln kobs[GdmCl] to the lower concentration of GdmCl, (iv) AMIMCl shifts the refolding and unfolding limps to vertically downwards and upwards, respectively, and (v) AMIMCl reducing the unfolding free energy estimated by both thermodynamic and kinetic analysis.
date: 2022-11
date_type: published
publication: BIOPHYSICAL CHEMISTRY
volume: 290
publisher: Elsevier
refereed: TRUE
official_url: https://www.sciencedirect.com/science/article/abs/pii/S030146222200134X?via%3Dihub
citation:   Garg, Mansi and Sharma, Deepak and Kumar, Rajesh  (2022) Analysis of the effect of 1-Allyl-3-Methylimidazolium chloride on thermodynamic stability, folding kinetics, and motional dynamics of horse cytochrome c.  BIOPHYSICAL CHEMISTRY, 290.