…
comment: The repository administrator has not yet configured an RDF license.
primary topic: L-asparaginase from Bacillus flexus strain SS: Isolation, Screening, Production Process Optimization, Purification, and Anticancer Activity

L-asparaginase from Bacillus flexus strain SS: Isolation, Screening, Production Process Optimization, Purification, and Anticancer Activity
type: AcademicArticle
type: Article
abstract: L-asparaginase has been widely accepted as a standard anticancer drug for acute lymphoblastic leukaemia (ALL). Presently in L-asparaginase biotherapeutic applications, the main focus is developing new L-asparaginase with minimal or without any glutaminase activity to reduce the associated adverse drug reactions. In this study, Bacillus flexus strain SS (NCBI GenBank Accession Number MN420983) was identified as a promising producer of L-asparaginase. L-asparaginase production was optimized by response surface methodology statistical bioprocess modelling, and enzyme yield of 25.08 IU/mL was reached at the bioreactor scale. The purification of L-asparaginase included ammonium sulfate precipitation, ion-exchange chromatography and size exclusion chromatography yielding 5.27-fold purification. The SDS-PAGE and CE-SDS revealed the monomeric L-asparaginase with molecular weight of 33 kDa. The purified enzyme was highly specific to substrate L-Asn and free from glutaminase activity. The anticancer activity of purified L-asparaginase was found specific against tumor cell lines SKBR3 (IC50 = 0.8 µg/mL), WIL2-S (IC50 = 16.2 µg/mL), and TF-1 (IC50 = 47 µg/mL), but not against negative control HUVEC cells. Therefore, L-asparaginase from B. flexus SS with no glutaminase activity could be a potential new candidate for anticancer drug for ALL with reduced adverse effects.
date: 2022-07-12
volume: 58
issue: 4
status: peerReviewed
status: published
author list: authors
type: EPrint
type: ArticleEPrint
is part of: repository
subject: QR Microbiology

Springer
type: Organization

APPLIED BIOCHEMISTRY AND MICROBIOLOGY
type: Collection

K. N. Mihooliya
type: Person
given name: K. N.
family name: Mihooliya

S. Chand
type: Person
given name: S.
family name: Chand

G. Sharma
type: Person
given name: G.
family name: Sharma

D. K. Sahoo
type: Person
given name: D. K.
family name: Sahoo

J. P. Prasad
type: Person
given name: J. P.
family name: Prasad

HTML Summary of #3055 L-asparaginase from Bacillus flexus strain SS: Isolation, Screening, Production Process Optimization, Purification, and Anticancer Activity
format: text/html
primary topic: L-asparaginase from Bacillus flexus strain SS: Isolation, Screening, Production Process Optimization, Purification, and Anticancer Activity

QR Microbiology
type: Concept