<> "The repository administrator has not yet configured an RDF license."^^ . <> . . . "L-asparaginase from Bacillus flexus strain SS: Isolation, Screening, Production Process Optimization, Purification, and Anticancer Activity"^^ . "L-asparaginase has been widely accepted as a standard anticancer drug for acute lymphoblastic leukaemia (ALL). Presently in L-asparaginase biotherapeutic applications, the main focus is developing new L-asparaginase with minimal or without any glutaminase activity to reduce the associated adverse drug reactions. In this study, Bacillus flexus strain SS (NCBI GenBank Accession Number MN420983) was identified as a promising producer of L-asparaginase. L-asparaginase production was optimized by response surface methodology statistical bioprocess modelling, and enzyme yield of 25.08 IU/mL was reached at the bioreactor scale. The purification of L-asparaginase included ammonium sulfate precipitation, ion-exchange chromatography and size exclusion chromatography yielding 5.27-fold purification. The SDS-PAGE and CE-SDS revealed the monomeric L-asparaginase with molecular weight of 33 kDa. The purified enzyme was highly specific to substrate L-Asn and free from glutaminase activity. The anticancer activity of purified L-asparaginase was found specific against tumor cell lines SKBR3 (IC50 = 0.8 µg/mL), WIL2-S (IC50 = 16.2 µg/mL), and TF-1 (IC50 = 47 µg/mL), but not against negative control HUVEC cells. Therefore, L-asparaginase from B. flexus SS with no glutaminase activity could be a potential new candidate for anticancer drug for ALL with reduced adverse effects."^^ . "2022-07-12" . "58" . "4" . . "Springer "^^ . . . "APPLIED BIOCHEMISTRY AND MICROBIOLOGY"^^ . . . . . . . . . . . . . . . . . . . . "K. N."^^ . "Mihooliya"^^ . "K. N. Mihooliya"^^ . . "S."^^ . "Chand"^^ . "S. Chand"^^ . . "G."^^ . "Sharma"^^ . "G. Sharma"^^ . . "D. K."^^ . "Sahoo"^^ . "D. K. Sahoo"^^ . . "J. P."^^ . "Prasad"^^ . "J. P. Prasad"^^ . . . . . "HTML Summary of #3055 \n\nL-asparaginase from Bacillus flexus strain SS: Isolation, Screening, Production Process Optimization, Purification, and Anticancer Activity\n\n" . "text/html" . . . "QR Microbiology"@en . .