creators_name: Sarkar, Anindya
creators_name: Paul, Sathi
creators_name: Singh, Charandeep
creators_name: Chowdhury, Nilkanta
creators_name: Nag, Papri
creators_name: Das, Swarnava
creators_name: Kumar, Sahil
creators_name: Sharma, Anshul
creators_name: Das, Deepjyoti Kumar
creators_name: Dutta, Dipak
creators_name: Thakur, Krishan Gopal
creators_name: Bagchi, Angshuman
creators_name: Shriti, Surbhi
creators_name: Das, Kali P.
creators_name: Ringe, Rajesh P.
creators_name: Das, Sampa
type: article
datestamp: 2022-12-15 09:36:58
lastmod: 2022-12-15 09:36:58
metadata_visibility: show
title: A novel plant lectin, NTL-125, interferes with SARS-CoV-2 interaction with hACE2
ispublished: pub
subjects: PZ
subjects: QR
keywords: Molecular docking; Mutant variants, Narcissus tazetta lectin; SARS-CoV-2; Spike Protein
note: The copyroght of this article belongs to Elsevier
abstract: COVID-19 caused by SARS-CoV-2 virus has had profound impact on the world in the past two years. Intense research is going on to find effective drugs to combat the disease. Over the past year several vaccines were approved for immunization. But SARS-CoV-2 being an RNA virus is continuously mutating to generate new variants, some of which develop features of immune escape. This raised serious doubts over the long-term efficacy of the vaccines. We have identified a unique mannose binding plant lectin from Narcissus tazetta bulb, NTL-125, which effectively inhibits SARS-CoV-2 replication in Vero-E6 cell line. In silico docking studies revealed that NTL-125 has strong affinity to viral Spike RBD protein, preventing it from attaching to hACE2 receptor, the gateway to cellular entry. Binding analyses revealed that all the mutant variants of Spike protein also have stronger affinity for NTL-125 than hACE2. The unique α-helical tail of NTL-125 plays most important role in binding to RBD of Spike. NTL-125 also interacts effectively with some glycan moieties of S-protein in addition to amino acid residues adding to the binding strength. Thus, NTL-125 is a highly potential antiviral compound of natural origin against SARS-CoV-2 and may serve as an important therapeutic for management of COVID-19.
date: 2022-07-02
date_type: published
publication: VIRUS RESEARCH
volume: 315
publisher: Elsevier
refereed: TRUE
official_url: https://www.sciencedirect.com/science/article/pii/S016817022200096X?via%3Dihub
citation:   Sarkar, Anindya and Paul, Sathi and Singh, Charandeep and Chowdhury, Nilkanta and Nag, Papri and Das, Swarnava and Kumar, Sahil and Sharma, Anshul and Das, Deepjyoti Kumar and Dutta, Dipak and Thakur, Krishan Gopal and Bagchi, Angshuman and Shriti, Surbhi and Das, Kali P. and Ringe, Rajesh P. and Das, Sampa  (2022) A novel plant lectin, NTL-125, interferes with SARS-CoV-2 interaction with hACE2.  VIRUS RESEARCH, 315.