%0 Journal Article
%A Das, Deepjyoti Kumar
%A Zafar, Mohammad Adeel
%A Nanda, Sidhanta
%A Singh, Sanpreet
%A Lamba, Taruna
%A Bashir, Hilal
%A Singh, Pargat
%A Maurya, Sudeep Kumar
%A Nadeem, Sajid
%A Sehrawat, Sharvan
%A Bhalla, Vijayender
%A Agrewala, Javed Naim
%D 2022
%F open:3072
%I Elsevier 
%J JOURNAL OF BIOLOGICAL CHEMISTRY
%K Mycobacterium tuberculosis; nanotechnology; peptides; toll-like receptor-2; vaccine.
%N 12
%T Targeting dendritic cells with TLR-2 ligand-coated nanoparticles loaded with Mycobacterium tuberculosis epitope induce antituberculosis immunity
%U http://crdd.osdd.net/open/3072/
%V 298
%X ovel vaccination strategies are crucial to efficiently control tuberculosis, as proposed by the World Health Organization under its flagship program “End TB Strategy.” However, the emergence of drug-resistant strains of Mycobacterium tuberculosis (Mtb), particularly in those coinfected with HIV-AIDS, constitutes a major impediment to achieving this goal. We report here a novel vaccination strategy that involves synthesizing a formulation of an immunodominant peptide derived from the Acr1 protein of Mtb. This nanoformulation in addition displayed on the surface a toll-like receptor-2 ligand to offer to target dendritic cells (DCs). Our results showed an efficient uptake of such a concoction by DCs in a predominantly toll-like receptor-2–dependent pathway. These DCs produced elevated levels of nitric oxide, proinflammatory cytokines interleukin-6, interleukin-12, and tumor necrosis factor-α, and upregulated the surface expression of major histocompatibility complex class II molecules as well as costimulatory molecules such as CD80 and CD86. Animals injected with such a vaccine mounted a significantly higher response of effector and memory Th1 cells and Th17 cells. Furthermore, we noticed a reduction in the bacterial load in the lungs of animals challenged with aerosolized live Mtb. Therefore, our findings indicated that the described vaccine triggered protective anti-Mtb immunity to control the tuberculosis infection.
%Z The copyright of this article belongs to Elsevier.