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primary topic: New carbohydrate binding domains identified by phage display based functional metagenomic screens of human gut microbiota.

New carbohydrate binding domains identified by phage display based functional metagenomic screens of human gut microbiota.
type: AcademicArticle
type: Article
abstract: Uncultured microbes represent a huge untapped biological resource of novel genes and gene products. Although recent genomic and metagenomic sequencing efforts have led to the identification of numerous genes that are homologous to existing annotated genes, there remains, yet, an enormous pool of unannotated genes that do not find significant sequence homology to existing annotated genes. Functional metagenomics offers a way to identify and annotate novel gene products. Here, we use functional metagenomics to mine novel carbohydrate binding domains that might aid human gut commensals in adherence, gut colonization, and metabolism of complex carbohydrates. We report the construction and functional screening of a metagenomic phage display library from healthy human fecal samples against dietary, microbial and host polysaccharides/glycoconjugates. We identify several protein sequences that do not find a hit to any known protein domain but are predicted to contain carbohydrate binding module-like folds. We heterologously express, purify and biochemically characterize some of these protein domains and demonstrate their carbohydrate-binding function. Our study reveals several previously unannotated carbohydrate-binding domains, including a levan binding domain and four complex N-glycan binding domains that might be useful for the labeling, visualization, and isolation of these glycans.
date: 2023-04-05
volume: 6
issue: 1
is part of: Nature communications
status: peerReviewed
status: published
author list: authors
type: EPrint
type: ArticleEPrint
is part of: repository
subject: QR Microbiology

Nature Pub. Group
type: Organization

Nature communications
type: Collection
issn: 20411723

Amit Kumar Yadav
type: Person
given name: Amit Kumar
family name: Yadav

T N C Ramya
type: Person
given name: T N C
family name: Ramya

Kajal Lnu
type: Person
given name: Kajal
family name: Lnu

Srikrishna Subramanian
type: Person
given name: Srikrishna
family name: Subramanian

Madhu Lata
type: Person
given name: Madhu
family name: Lata

Akil Akhtar
type: Person
given name: Akil
family name: Akhtar

Sonali Sunsunwal
type: Person
given name: Sonali
family name: Sunsunwal

HTML Summary of #3078 New carbohydrate binding domains identified by phage display based functional metagenomic screens of human gut microbiota.
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primary topic: New carbohydrate binding domains identified by phage display based functional metagenomic screens of human gut microbiota.

QR Microbiology
type: Concept