TY  - JOUR
ID  - open309
UR  - http://www.sciencedirect.com/science/article/pii/S0006291X00932169
IS  - 1
A1  - Bhushan, B
A1  - Samanta, S K
A1  - Chauhan, Archana
A1  - Chakraborti, Asit K
A1  - Jain, R K
N2  - 3-Methyl-4-nitrophenol is one of the major breakdown products of fenitrothion [O,O-dimethyl O-(3-methyl-4-nitrophenyl) thiophosphate], a recalcitrant organophosphate insecticide used in agriculture. Being the non-polar methylated aromatic compound, 3-methyl-4-nitrophenol is highly toxic and, therefore, a complete degradation of this compound is important for environmental decontamination/bioremediation purposes. A gram negative, motile Ralstonia sp. SJ98 was isolated by selective screening from a soil sample contaminated with pesticides. The microorganism was capable of utilizing 3-methyl-4-nitrophenol as the sole source of carbon and energy. Thin layer chromatography (TLC), gas chromatography (GC), gas chromatography-mass spectrometry (GC-MS), and high performance liquid chromatography (HPLC) were performed to determine the possible intermediates in the degradative pathway of this compound. Taken together, catechol was found to be one of the major intermediate of the pathway. Furthermore, the chemotactic behavior of Ralstonia sp. SJ98 towards 3-methyl-4-nitrophenol was tested using three different methods i.e., drop assay, swarm plate assay and capillary assay, which were found to be positive towards this compound. This is the first report clearly indicating the involvement of a microorganism in the chemotaxis and biodegradation of methyl-4-nitrophenol and formation of catechol as an intermediate in the degradative pathway.
VL  - 275
TI  - Chemotaxis and biodegradation of 3-methyl- 4-nitrophenol by Ralstonia sp. SJ98.
AV  - restricted
EP  - 33
N1  - Copyright of this article belongs to Elsevier Science.
Y1  - 2000/08/18/
PB  - Elsevier Science
JF  - Biochemical and biophysical research communications
KW  - Ralstonia sp. SJ98; 3-methyl-4-nitrophenol degradation; catechol formation; chemotaxis
SN  - 0006-291X
SP  - 129
ER  -