creators_name: Mavi, Parminder Singh
creators_name: Singh, Shweta
creators_name: Kumar, Ashwani
type: article
datestamp: 2023-06-07 11:23:54
lastmod: 2023-06-07 11:23:54
metadata_visibility: show
title: Media component bovine serum albumin facilitates the formation of mycobacterial biofilms in response to reductive stress

ispublished: pub
subjects: QR
keywords: Bovine serum albumin; Cellulose sensor; Drug tolerance; IMT-CBD-mC; Mycobacterial biofilms; Mycobacterium; Reductive stress; Thiol-reductive stress.
note: The copyright of this article belongs to Bio Med Central
abstract: Background: Mycobacterium tuberculosis (Mtb) forms physiologically relevant biofilms harboring drug-tolerant bacteria. This observation has brought the study of mycobacterial biofilms to the forefront of tuberculosis research. We established earlier that dithiothreitol (DTT) mediated reductive stress induces cellulose-rich biofilm formation in Mtb cultures. The molecular events associated with the DTT-induced biofilm formation are not known. Furthermore, there are only limited tools for monitoring the presence of cellulose in biofilms.

Results: To decipher the molecular events associated with DTT-induced biofilm formation, we used Mtb and non-pathogenic, fast-growing Mycobacterium smegmatis (Msm). We observed that DTT induces biofilm formation in Msm cultures. We explored whether media components facilitate biofilm formation in mycobacteria upon exposure to DTT. We observed that media component bovine serum albumin promotes mycobacterial biofilm formation in response to DTT. Furthermore, we analyzed the composition of extracellular polymeric substances of Msm biofilms. We found that, like Mtb biofilms, Msm biofilms are also rich in polysaccharides and proteins. We also developed a novel protein-based molecular probe for imaging cellulose by utilizing the cellulose-binding domain of cellulase CenA from Cellulomonas fimi and fusing it to fluorescent reporter mCherry. Characterization of this new probe revealed that it has a high affinity for cellulose and could be used for visualizing cellulose biosynthesis during the development of Agrobacterium biofilms. Furthermore, we have demonstrated that biological macromolecule cellulose is present in the extracellular polymeric substances of Msm biofilms using this novel probe.


date: 2023-04-20
date_type: published
publication: BMC microbiology
volume: 23
number: 1
publisher: BioMedCentral
refereed: TRUE
issn: 1471-2180
official_url: https://https://bmcmicrobiol.biomedcentral.com/articles/10.1186/s12866-023-02853-6
citation:   Mavi, Parminder Singh and Singh, Shweta and Kumar, Ashwani  (2023) Media component bovine serum albumin facilitates the formation of mycobacterial biofilms in response to reductive stress.  BMC microbiology, 23 (1).   ISSN 1471-2180