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primary topic: The physiological effect of rimI/rimJ silencing by CRISPR interference in Mycobacterium smegmatis mc2155

The physiological effect of rimI/rimJ silencing by CRISPR interference in Mycobacterium smegmatis mc2155
type: AcademicArticle
type: Article
abstract: N-terminal acetylation of proteins is an important post-translational modification (PTM) found in eukaryotes and prokaryotes. In bacteria, N-terminal acetylation is suggested to play various regulatory roles related to protein stability, gene expression, stress response, and virulence; however, the mechanism of such response remains unclear. The proteins, namely RimI/RimJ, are involved in N-terminal acetylation in mycobacteria. In this study, we used CRISPR interference (CRISPRi) to silence rimI/rimJ in Mycobacterium smegmatis mc2155 to investigate the physiological effects of N-terminal acetylation in cell survival and stress response. Repeat analysis of growth curves in rich media and biofilm analysis in minimal media of various mutant strains and wild-type bacteria did not show significant differences that could be attributed to the rimI/rimJ silencing. However, total proteome and acetylome profiles varied significantly across mutants and wild-type strains, highlighting the role of RimI/RimJ in modulating levels of proprotein acetylation in the cellular milieu. Further, we observed a significant increase in the minimum inhibitory concentration (MIC) (from 64 to 1024 µg ml-1) for the drug isoniazid in rimI mutant strains. The increase in MIC value for the drug isoniazid in the mutant strains suggests the link between N-terminal acetylation and antibiotic resistance. The study highlights the utility of CRISPRi as a convenient tool to study the role of PTMs, such as acetylation in mycobacteria. It also identifies rimI/rimJ genes as necessary for managing cellular response against antibiotic stress. Further research would be required to decipher the potential of targeting acetylation to enhance the efficacy of existing antibiotics.
date: 2023-04-29
volume: 205
issue: 5
is part of: Archives of microbiology
status: peerReviewed
status: published
author list: authors
type: EPrint
type: ArticleEPrint
is part of: repository
subject: QR Microbiology

Springer Science
type: Organization

Archives of microbiology
type: Collection
issn: 03028933

Pramila Pal
type: Person
given name: Pramila
family name: Pal

Nisheeth Agarwal
type: Person
given name: Nisheeth
family name: Agarwal

Mohinder Pal
type: Person
given name: Mohinder
family name: Pal

Alka Rao
type: Person
given name: Alka
family name: Rao

Kumar Vinay Yadav
type: Person
given name: Kumar Vinay
family name: Yadav

HTML Summary of #3099 The physiological effect of rimI/rimJ silencing by CRISPR interference in Mycobacterium smegmatis mc2155
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primary topic: The physiological effect of rimI/rimJ silencing by CRISPR interference in Mycobacterium smegmatis mc2155

QR Microbiology
type: Concept