TY  - JOUR
N1  - The copyright of this article belongs to Springer Science
ID  - open3099
UR  - https://https://link.springer.com/article/10.1007/s00203-023-03561-5
IS  - 5
A1  - Pal, Mohinder
A1  - Yadav , Kumar Vinay
A1  - Pal, Pramila
A1  - Agarwal, Nisheeth
A1  - Rao, Alka
Y1  - 2023/04/29/
N2  - N-terminal acetylation of proteins is an important post-translational modification (PTM) found in eukaryotes and prokaryotes. In bacteria, N-terminal acetylation is suggested to play various regulatory roles related to protein stability, gene expression, stress response, and virulence; however, the mechanism of such response remains unclear. The proteins, namely RimI/RimJ, are involved in N-terminal acetylation in mycobacteria. In this study, we used CRISPR interference (CRISPRi) to silence rimI/rimJ in Mycobacterium smegmatis mc2155 to investigate the physiological effects of N-terminal acetylation in cell survival and stress response. Repeat analysis of growth curves in rich media and biofilm analysis in minimal media of various mutant strains and wild-type bacteria did not show significant differences that could be attributed to the rimI/rimJ silencing. However, total proteome and acetylome profiles varied significantly across mutants and wild-type strains, highlighting the role of RimI/RimJ in modulating levels of proprotein acetylation in the cellular milieu. Further, we observed a significant increase in the minimum inhibitory concentration (MIC) (from 64 to 1024 µg ml-1) for the drug isoniazid in rimI mutant strains. The increase in MIC value for the drug isoniazid in the mutant strains suggests the link between N-terminal acetylation and antibiotic resistance. The study highlights the utility of CRISPRi as a convenient tool to study the role of PTMs, such as acetylation in mycobacteria. It also identifies rimI/rimJ genes as necessary for managing cellular response against antibiotic stress. Further research would be required to decipher the potential of targeting acetylation to enhance the efficacy of existing antibiotics.


PB  - Springer Science
JF  - Archives of microbiology
VL  - 205
KW  - CRISPRi; Drug resistance; Minimum inhibitory concentration; Mycobacterium; N-acetylation.
SN  - 0302-8933
TI  - The physiological effect of rimI/rimJ silencing by CRISPR interference in Mycobacterium smegmatis mc2155

ER  -