creators_name: Rezgui, Raja
creators_name: Walia, Ruhi
creators_name: Sharma, Jyoti
creators_name: Sidhu, Dwinder
creators_name: Alshagadali, Khalid
creators_name: Chaudhuri, Saumya Ray
creators_name: Saeed, Amir
creators_name: Dey, Priyankar
type: article
datestamp: 2023-06-07 12:40:45
lastmod: 2023-06-07 12:40:45
metadata_visibility: show
title: Chemically Defined Lactobacillus plantarum Cell-Free Metabolites Demonstrate Cytoprotection in HepG2 Cells through Nrf2-Dependent Mechanism

ispublished: pub
subjects: QR
keywords: Lactobacillus; Nrf2; antioxidant; inflammation; liver; metabolites; oxidative stress; probiotic.
note: The copyright of this article belongs to MDPI 
abstract: Centering around the concept that metabolites from the gut commensals can exert metabolic health benefits along the gut-liver axis, we tested whether the cell-free global metabolome of probiotic bacteria can exert hepatoprotective benefits against H2O2-induced oxidative stress. Cell-free global metabolites of Lactobacillus plantarum (LPM) were isolated and untargeted metabolomics was performed. The free radical scavenging potentials of LPM were measured. The cytoprotective effects of LPM were tested on HepG2 cells. A total of 66 diverse metabolites were identified in LPM, among which saturated fatty acids, amino acids and dicarboxylic acids were highly enriched. LPM attenuated cell damage, lipid peroxidation and the levels of intracellular cytoprotective enzymes in H2O2-treated cells. LPM also attenuated H2O2-induced increased expressions of TNF-α and IL-6. However, the cytoprotective effects of LPM were diminished in cells that were pretreated with a pharmacological inhibitor of Nrf2. Our data collectively indicate that LPM can significantly attenuate oxidative damage to HepG2 cells. However, the cytoprotective effects of LPM likely depend on an Nrf2-dependent mechanism.


date: 2023-04-14
date_type: published
publication: Antioxidants
volume: 12
number: 4
publisher: MDPI
refereed: TRUE
issn: 1557-7716
official_url: https://doi.org/10.3390/antiox12040930
citation:   Rezgui, Raja and Walia, Ruhi and Sharma, Jyoti and Sidhu, Dwinder and Alshagadali, Khalid and Chaudhuri, Saumya Ray and Saeed, Amir and Dey, Priyankar  (2023) Chemically Defined Lactobacillus plantarum Cell-Free Metabolites Demonstrate Cytoprotection in HepG2 Cells through Nrf2-Dependent Mechanism.  Antioxidants, 12 (4).   ISSN 1557-7716