title: Studies on a new antimicrobial peptide from Vibrio proteolyticus MT110
creator: Verma, Himanshu
creator: Mihooliya, Kanti N.
creator: Nandal, Jitender
creator: Sahoo, Debendra K.
subject: QR Microbiology
description: The marine environment is known for its vast diversity of the microbial population; however, less explored for bioactive compounds. In this study, an AMP produced by a new marine isolate, Vibrio proteolyticus MT110, showed broad-spectrum antimicrobial activity against Gram-positive and Gram-negative bacteria. The AMP was purified to homogeneity using ethyl acetate extraction followed by RP-HPLC, and LC-MS analysis showed its molecular weight as 980 Da. The MIC of AMP (peptide-MT110) was obtained in the 7.81-31.25 µg/mL range against different indicator strains. Peptide-MT110 showed stability of its antimicrobial activity at 15-121 °C and pH 4-10 and in the presence of various hydrolytic enzymes. The peaks at 1536 cm-1 and 1712 cm-1 wavenumbers in FTIR spectra confirmed the peptidic nature of AMP, and its amino acid analysis confirmed the presence of tyrosine and isoleucine. The antibacterial activity of peptide-MT110 is confirmed by PI assay and TEM. The optimization of peptide-MT110 production using statistical methods resulted in a 2.64-fold higher production. The physicochemical properties and stability in wide pH and temperature ranges showed the potential of peptide-MT110 for its development as a drug candidate. This is believed to be the first report on an AMP from Vibrio proteolyticus.
publisher: TAYLOR AND FRANCIS
date: 2024-02
type: Article
type: PeerReviewed
relation: https://www.tandfonline.com/doi/full/10.1080/10826068.2023.2209892
identifier:   Verma, Himanshu and Mihooliya, Kanti N. and Nandal, Jitender and Sahoo, Debendra K.  (2024) Studies on a new antimicrobial peptide from Vibrio proteolyticus MT110.  PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY, 54 (2).  pp. 193-206.      
relation: http://crdd.osdd.net/open/3107/