creators_name: Verma, Neha
creators_name: Sharma, Tina
creators_name: Bhardwaj, Anshu
creators_name: Vemuluri, Venkata Ramana
type: article
datestamp: 2024-06-24 03:09:00
lastmod: 2024-06-24 03:09:00
metadata_visibility: show
title: Comparative genomics and characterization of a multidrug-resistant Acinetobacter baumannii VRL-M19 isolated from a crowded setting in India
ispublished: pub
subjects: QR
keywords:  Acinetobacter baumannii; Antimicrobial resistance; Mass gathering; Pangenome; Virulence factors.
note: The copyright of this article belongs to ELSEVIER
abstract: A crowded vegetable market serves as a mass gathering, posing a potential risk for infection transmission. In this study, we isolated a multidrug-resistant Acinetobacter baumannii strain, VRL-M19, from the air of such a market and conducted comparative genomics and phenotypic characterization. Antimicrobial susceptibility testing, genome sequencing using Illumina HiSeq X10, and pan-genome analysis with 788 clinical isolates identified core, accessory, and unique drug-resistant determinants. Mutational analysis of drug-resistance genes, virulence factor annotation, in vitro pathogenicity assessment, subsystem analysis, Multilocus sequence typing, and whole genome phylogenetic analysis were performed. VRL-M19 exhibited multidrug resistance with 69 determinants, and analysis across 788 clinical isolates and 350 Indian isolates revealed more accessory genes (52 out of 69) in the Indian isolates. Multiple mutations were observed in drug target modification genes, and the strain was identified as a moderate biofilm-former with 55 virulence factors. Whole genome phylogenetics indicated a close relationship between VRL-M19 and clinical A. baumannii strains. In conclusion, our comprehensive study suggests that VRL-M19 is a multidrug-resistant, potential pathogen with biofilm-forming capabilities, closely associated with clinical A. baumannii strains.
date: 2024-03
date_type: published
publication: INFECTION GENETICS AND EVOLUTION
volume: 118
publisher: ELSEVIER 
id_number: 10.1016/j.meegid.2023.105549
refereed: TRUE
official_url: https://www.sciencedirect.com/science/article/pii/S1567134823001478?via%3Dihub
citation:   Verma, Neha and Sharma, Tina and Bhardwaj, Anshu and Vemuluri, Venkata Ramana  (2024) Comparative genomics and characterization of a multidrug-resistant Acinetobacter baumannii VRL-M19 isolated from a crowded setting in India.  INFECTION GENETICS AND EVOLUTION, 118.