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primary topic: A sucrose non-fermenting-1-related protein kinase 1 gene from wheat, TaSnRK1α regulates starch biosynthesis by modulating AGPase activity

A sucrose non-fermenting-1-related protein kinase 1 gene from wheat, TaSnRK1α regulates starch biosynthesis by modulating AGPase activity
type: AcademicArticle
type: Article
abstract: Major portion of wheat grain consist of carbohydrate, mainly starch. The proportion of amylose and amylopectin in starch greatly influence the end product quality. Advancement in understanding starch biosynthesis pathway and modulating key genes has enabled the genetic modification of crops resulting in enhanced starch quality. However, the regulation of starch biosynthesis genes still remains unexplored. So, to expand the limited knowledge, here, we characterized a Ser/Thr kinase, SnRK1α in wheat and determined its role in regulating starch biosynthesis. SnRK1 is an evolutionary conserved protein kinase and share homology to yeast SNF1. Yeast complementation assay suggests TaSnRK1α restores growth defect and promotes glycogen accumulation. Domain analysis and complementation assay with truncated domain proteins suggest the importance of ATP-binding and UBA domain in TaSnRK1α activity. Sub-cellular localization identified nuclear and cytoplasmic localization of TaSnRK1α in tobacco leaves. Further, heterologous over-expression (O/E) of TaSnRK1α in Arabidopsis not only led to increase in starch content but also enlarges the starch granules. TaSnRK1α was found to restore starch accumulation in Arabidopsis kin10. Remarkably, TaSnRK1α O/E increases the AGPase activity suggesting the direct regulation of rate limiting enzyme AGPase involved in starch biosynthesis. Furthermore, in vitro and in vivo interaction assay reveal that TaSnRK1α interacts with AGPase large sub-unit. Overall, our findings indicate that TaSnRK1α plays a role in starch biosynthesis by regulating AGPase activity.
date: 2024-02
volume: 207
status: peerReviewed
status: published
author list: authors
type: EPrint
type: ArticleEPrint
is part of: repository
subject: QR Microbiology

ELSEVIER
type: Organization

PLANT PHYSIOLOGY AND BIOCHEMISTRY
type: Collection

Joy Roy
type: Person
given name: Joy
family name: Roy

Akansha Madhawan
type: Person
given name: Akansha
family name: Madhawan

Prashant Kumar
type: Person
given name: Prashant
family name: Kumar

Vinita Sharma
type: Person
given name: Vinita
family name: Sharma

Vikas Fandade
type: Person
given name: Vikas
family name: Fandade

Deepak Sharma
type: Person
given name: Deepak
family name: Sharma

Akshya Sharma
type: Person
given name: Akshya
family name: Sharma

Deepak Das
type: Person
given name: Deepak
family name: Das

Afsana Parveen
type: Person
given name: Afsana
family name: Parveen

HTML Summary of #3128 A sucrose non-fermenting-1-related protein kinase 1 gene from wheat, TaSnRK1α regulates starch biosynthesis by modulating AGPase activity
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primary topic: A sucrose non-fermenting-1-related protein kinase 1 gene from wheat, TaSnRK1α regulates starch biosynthesis by modulating AGPase activity

QR Microbiology
type: Concept