%0 Journal Article
%A Negi, Sushmita
%A Tandel, Nikunj
%A Garg, Neeraj K.
%A Sharma, Prakriti
%A Kumar, Rajinder
%A Sharma, Praveen
%A Kumar, Reetesh
%A Saini, Sheetal
%A Sharma, Aman
%A Tyagi, Rajeev K.
%D 2024
%F open:3140
%I DOVE PRESS
%J INTERNATIONAL JOURNAL OF NANOMEDICINE
%K  MMP-1; aceclofenac; lipid polymer hybrid nanoparticles; methotrexate; nanostructured lipid nanocarriers; rheumatoid arthritis.
%P 2149-2177
%T Co-Delivery of Aceclofenac and Methotrexate Nanoparticles Presents an Effective Treatment for Rheumatoid Arthritis
%U http://crdd.osdd.net/open/3140/
%V 19
%X Background: Rheumatoid arthritis (RA) is a common acute inflammatory autoimmune connective tissue arthropathy. The genetic studies, tissue analyses, experimental animal models, and clinical investigations have confirmed that stromal tissue damage and pathology driven by RA mounts the chronic inflammation and dysregulated immune events.    Methods: We developed methotrexate (MTX)-loaded lipid-polymer hybrid nanoparticles (MTX-LPHNPs) and aceclofenac (ACE)-loaded nanostructured lipid carriers (ACE-NLCs) for the efficient co-delivery of MTX and ACE via intravenous and transdermal routes, respectively. Bio-assays were performed using ex-vivo skin permeation and transport, macrophage model of inflammation (MMI) (LPS-stimulated THP-1 macrophages), Wistar rats with experimental RA (induction of arthritis with Complete Freund's adjuvant; CFA and BCG), and programmed death of RA affected cells. In addition, gene transcription profiling and serum estimation of inflammatory, signaling, and cell death markers were performed on the blood samples collected from patients with RA.
%Z The copyright of this article belongs to DOVE PRESS