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primary topic: RUFY1 binds Arl8b and mediates endosome-to-TGN CI-M6PR retrieval for cargo sorting to lysosomes

RUFY1 binds Arl8b and mediates endosome-to-TGN CI-M6PR retrieval for cargo sorting to lysosomes
type: AcademicArticle
type: Article
abstract: Arl8b, an Arf-like GTP-binding protein, regulates cargo trafficking and positioning of lysosomes. However, it is unknown whether Arl8b regulates lysosomal cargo sorting. Here, we report that Arl8b binds to the Rab4 and Rab14 interaction partner, RUN and FYVE domain-containing protein (RUFY) 1, a known regulator of cargo sorting from recycling endosomes. Arl8b determines RUFY1 endosomal localization through regulating its interaction with Rab14. RUFY1 depletion led to a delay in CI-M6PR retrieval from endosomes to the TGN, resulting in impaired delivery of newly synthesized hydrolases to lysosomes. We identified the dynein-dynactin complex as an RUFY1 interaction partner, and similar to a subset of activating dynein adaptors, the coiled-coil region of RUFY1 was required for interaction with dynein and the ability to mediate dynein-dependent organelle clustering. Our findings suggest that Arl8b and RUFY1 play a novel role on recycling endosomes, from where this machinery regulates endosomes to TGN retrieval of CI-M6PR and, consequently, lysosomal cargo sorting.
date: 2022-10-25
volume: 222
issue: 1
status: peerReviewed
status: published
author list: authors
type: EPrint
type: ArticleEPrint
is part of: repository
subject: QR Microbiology

ROCKFELLER UNIVERSITY PRESS
type: Organization

Journal of Cell Biology
type: Collection

Gitanjali Charak
type: Person
given name: Gitanjali
family name: Charak

Cecilia De Heus
type: Person
given name: Cecilia De
family name: Heus

Divya Khatter
type: Person
given name: Divya
family name: Khatter

Klumperman Judith
type: Person
given name: Klumperman
family name: Judith

Sheetal Sharma
type: Person
given name: Sheetal
family name: Sharma

Shalini Rawat
type: Person
given name: Shalini
family name: Rawat

Mahak Sharma
type: Person
given name: Mahak
family name: Sharma

Dhruba Chatterjee
type: Person
given name: Dhruba
family name: Chatterjee

Nalan Liv
type: Person
given name: Nalan
family name: Liv

Rituraj Marwaha
type: Person
given name: Rituraj
family name: Marwaha

Gaurav Kumar
type: Person
given name: Gaurav
family name: Kumar

Shrestha Shaw
type: Person
given name: Shrestha
family name: Shaw

Amit Tuli
type: Person
given name: Amit
family name: Tuli

HTML Summary of #3150 RUFY1 binds Arl8b and mediates endosome-to-TGN CI-M6PR retrieval for cargo sorting to lysosomes
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primary topic: RUFY1 binds Arl8b and mediates endosome-to-TGN CI-M6PR retrieval for cargo sorting to lysosomes

QR Microbiology
type: Concept