<> "The repository administrator has not yet configured an RDF license."^^ . <> . . . "RUFY1 binds Arl8b and mediates endosome-to-TGN CI-M6PR retrieval for cargo sorting to lysosomes"^^ . "Arl8b, an Arf-like GTP-binding protein, regulates cargo trafficking and positioning of lysosomes. However, it is unknown whether Arl8b regulates lysosomal cargo sorting. Here, we report that Arl8b binds to the Rab4 and Rab14 interaction partner, RUN and FYVE domain-containing protein (RUFY) 1, a known regulator of cargo sorting from recycling endosomes. Arl8b determines RUFY1 endosomal localization through regulating its interaction with Rab14. RUFY1 depletion led to a delay in CI-M6PR retrieval from endosomes to the TGN, resulting in impaired delivery of newly synthesized hydrolases to lysosomes. We identified the dynein-dynactin complex as an RUFY1 interaction partner, and similar to a subset of activating dynein adaptors, the coiled-coil region of RUFY1 was required for interaction with dynein and the ability to mediate dynein-dependent organelle clustering. Our findings suggest that Arl8b and RUFY1 play a novel role on recycling endosomes, from where this machinery regulates endosomes to TGN retrieval of CI-M6PR and, consequently, lysosomal cargo sorting.\r\n\r\n"^^ . "2022-10-25" . "222" . "1" . . "ROCKFELLER UNIVERSITY PRESS"^^ . . . "Journal of Cell Biology "^^ . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . "Gitanjali"^^ . "Charak"^^ . "Gitanjali Charak"^^ . . "Cecilia De"^^ . "Heus"^^ . "Cecilia De Heus"^^ . . "Divya"^^ . "Khatter"^^ . "Divya Khatter"^^ . . "Klumperman"^^ . "Judith "^^ . "Klumperman Judith "^^ . . "Sheetal"^^ . "Sharma"^^ . "Sheetal Sharma"^^ . . "Shalini"^^ . "Rawat"^^ . "Shalini Rawat"^^ . . "Mahak"^^ . "Sharma"^^ . "Mahak Sharma"^^ . . "Dhruba"^^ . "Chatterjee"^^ . "Dhruba Chatterjee"^^ . . "Nalan"^^ . "Liv"^^ . "Nalan Liv"^^ . . "Rituraj"^^ . "Marwaha"^^ . "Rituraj Marwaha"^^ . . "Gaurav"^^ . "Kumar"^^ . "Gaurav Kumar"^^ . . "Shrestha"^^ . "Shaw"^^ . "Shrestha Shaw"^^ . . "Amit"^^ . "Tuli"^^ . "Amit Tuli"^^ . . . . . "HTML Summary of #3150 \n\nRUFY1 binds Arl8b and mediates endosome-to-TGN CI-M6PR retrieval for cargo sorting to lysosomes\n\n" . "text/html" . . . "QR Microbiology"@en . .