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primary topic: Efflux pump inhibitory potential of indole derivatives as an arsenal against norA over-expressing Staphylococcus aureus

Efflux pump inhibitory potential of indole derivatives as an arsenal against norA over-expressing Staphylococcus aureus
type: AcademicArticle
type: Article
abstract: NorA, an extensively studied efflux pump in Staphylococcus aureus, has been connected to fluoroquinolone, antiseptic, and disinfection resistance. Several studies have also emphasized how efflux pumps, including NorA, function as the first line of defense of S. aureus against antibiotics. In this study, we have screened some chemically synthesized indole derivatives for their activity as efflux pump inhibitors (EPIs). The derivative SMJ-5 was found to be a potent NorA efflux pump inhibitor among the screened indole derivatives, owing to increased ethidium bromide and norfloxacin accumulation in norA over-expressing S. aureus. The combination of SMJ-5 and ciprofloxacin demonstrated the eradication of S. aureus biofilm and prolonged the post-antibiotic effect more than ciprofloxacin alone. SMJ-5 was able to inhibit staphyloxanthin virulence. In in vitro time-kill trials and in vivo efficacy investigations, the combination enhanced the bactericidal activity of ciprofloxacin against S. aureus. Additionally, reverse transcription PCR results revealed that SMJ-5 also inhibits the NorA efflux pump indirectly at the transcriptional level.
date: 2023-09-27
volume: 11
issue: 5
status: peerReviewed
status: published
author list: authors
type: EPrint
type: ArticleEPrint
is part of: repository
subject: QR Microbiology

ASM
type: Organization

Microbiology Spectrum
type: Collection

Gautam Kumar
type: Person
given name: Gautam
family name: Kumar

Nishtha Chandal
type: Person
given name: Nishtha
family name: Chandal

Sanjay Jachak
type: Person
given name: Sanjay
family name: Jachak

Rushikesh Tambat
type: Person
given name: Rushikesh
family name: Tambat

Nisha Mahey
type: Person
given name: Nisha
family name: Mahey

Ritu Kalia
type: Person
given name: Ritu
family name: Kalia

Hemraj Nandanwar
type: Person
given name: Hemraj
family name: Nandanwar

HTML Summary of #3167 Efflux pump inhibitory potential of indole derivatives as an arsenal against norA over-expressing Staphylococcus aureus
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primary topic: Efflux pump inhibitory potential of indole derivatives as an arsenal against norA over-expressing Staphylococcus aureus

QR Microbiology
type: Concept