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primary topic: Purification and characterization of extracellular lipase from a thermotolerant strain: Bacillus subtilis TTP-06

Purification and characterization of extracellular lipase from a thermotolerant strain: Bacillus subtilis TTP-06
type: AcademicArticle
type: Article
abstract: In current study, lipase from a thermotolerant Bacillus subtilis TTP-06 was purified in a stepwise manner by using ammonium sulfate precipitation and column chromatography. Thenceforth, it was subjected to sodium dodecyl sulfate- and native-polyacrylamide gel electrophoresis to check the homogeneity of the purified enzyme. The ideal substrate concentration, pH, temperature, reaction duration and lipase specificity were identified. With a yield of 11.02%, purified lipase displayed activity of 8.51 U/mg. Thenceforward, the homogeneously purified enzyme was considered to be a homo-dimer of 30 kDa subunits. Enzyme had Km and Vmax value of 9.498 mM and 19.92 mol mg-1 min-1, respectively. Additionally, the matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) method was used to investigate the purified lipase and estimate its 3-D structure, which revealed a catalytic triad of serine, aspartate and histidine.
date: 2023-10
volume: 13
issue: 10
is part of: 3 Biotech
status: peerReviewed
status: published
author list: authors
type: EPrint
type: ArticleEPrint
is part of: repository
subject: QR Microbiology

SPRINGER LINK
type: Organization

3 Biotech
type: Collection
issn: 2190572X

Poonam Katoch
type: Person
given name: Poonam
family name: Katoch

Rakesh Kumar
type: Person
given name: Rakesh
family name: Kumar

Reena Gupta
type: Person
given name: Reena
family name: Gupta

Manpreet Kaur
type: Person
given name: Manpreet
family name: Kaur

HTML Summary of #3182 Purification and characterization of extracellular lipase from a thermotolerant strain: Bacillus subtilis TTP-06
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primary topic: Purification and characterization of extracellular lipase from a thermotolerant strain: Bacillus subtilis TTP-06

QR Microbiology
type: Concept