<> "The repository administrator has not yet configured an RDF license."^^ . <> . . . "Purification and characterization of extracellular lipase from a thermotolerant strain: Bacillus subtilis TTP-06\r\n"^^ . "In current study, lipase from a thermotolerant Bacillus subtilis TTP-06 was purified in a stepwise manner by using ammonium sulfate precipitation and column chromatography. Thenceforth, it was subjected to sodium dodecyl sulfate- and native-polyacrylamide gel electrophoresis to check the homogeneity of the purified enzyme. The ideal substrate concentration, pH, temperature, reaction duration and lipase specificity were identified. With a yield of 11.02%, purified lipase displayed activity of 8.51 U/mg. Thenceforward, the homogeneously purified enzyme was considered to be a homo-dimer of 30 kDa subunits. Enzyme had Km and Vmax value of 9.498 mM and 19.92 mol mg-1 min-1, respectively. Additionally, the matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) method was used to investigate the purified lipase and estimate its 3-D structure, which revealed a catalytic triad of serine, aspartate and histidine.\r\n\r\n"^^ . "2023-10" . "13" . "10" . . "SPRINGER LINK"^^ . . . "3 Biotech"^^ . . . "2190572X" . . . . . . . . . . . . . . . . "Poonam"^^ . "Katoch"^^ . "Poonam Katoch"^^ . . "Rakesh "^^ . "Kumar"^^ . "Rakesh Kumar"^^ . . "Reena"^^ . "Gupta"^^ . "Reena Gupta"^^ . . "Manpreet"^^ . "Kaur "^^ . "Manpreet Kaur "^^ . . . . . "HTML Summary of #3182 \n\nPurification and characterization of extracellular lipase from a thermotolerant strain: Bacillus subtilis TTP-06 \n\n\n" . "text/html" . . . "QR Microbiology"@en . .