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primary topic: Enhancing Immunogenicity of a Thermostable, Efficacious SARS-CoV-2 Vaccine Formulation through Oligomerization and Adjuvant Choice

Enhancing Immunogenicity of a Thermostable, Efficacious SARS-CoV-2 Vaccine Formulation through Oligomerization and Adjuvant Choice
type: AcademicArticle
type: Article
abstract: Currently deployed SARS-CoV-2 vaccines all require storage at refrigerated or sub-zero temperatures. We demonstrate that after month-long incubation at 37 °C, solubilization, and formulation with squalene-in-water emulsion adjuvant, a stabilized receptor binding domain retains immunogenicity and protective efficacy. We also examine the effects of trimerization of the stabilized RBD, as well as of additional adjuvants, on both B and T-cell responses. The additional emulsion or liposome-based adjuvants contained a synthetic TLR-4 ligand and/or the saponin QS-21. Trimerization enhanced immunogenicity, with significant antibody titers detectable after a single immunization. Saponin-containing adjuvants elicited enhanced immunogenicity relative to both emulsion and aluminum hydroxide adjuvanted formulations lacking these immunostimulants. Trimeric RBD formulated with liposomal based adjuvant containing both TLR-4 ligand and saponin elicited a strongly Th1 biased response, with ~10-fold higher neutralization titers than the corresponding aluminum hydroxide adjuvanted formulation. The SARS-CoV-2 virus is now endemic in humans, and it is likely that periodic updating of vaccine formulations in response to viral evolution will continue to be required to protect vulnerable individuals. In this context, it is desirable to have efficacious, thermostable vaccine formulations to facilitate widespread vaccine coverage, including in low- and middle-income countries, where global access rights to clinically de-risked adjuvants will be important moving forward.
date: 2023-12
volume: 15
issue: 12
status: peerReviewed
status: published
author list: authors
type: EPrint
type: ArticleEPrint
is part of: repository
subject: QR Microbiology

MDPI
type: Organization

Pharmaceutics
type: Collection

Mohammad Suhail Khan
type: Person
given name: Mohammad Suhail
family name: Khan

Rajesh P. Ringe
type: Person
given name: Rajesh P.
family name: Ringe

Randhir Singh
type: Person
given name: Randhir
family name: Singh

Raghavan Varadarajan
type: Person
given name: Raghavan
family name: Varadarajan

Virginie Jakob
type: Person
given name: Virginie
family name: Jakob

Celine Lemonie
type: Person
given name: Celine
family name: Lemonie

Patrice M Dubois
type: Person
given name: Patrice M
family name: Dubois

Harry Kleanthous
type: Person
given name: Harry
family name: Kleanthous

Raju S Rajmani
type: Person
given name: Raju S
family name: Rajmani

Sahil Kumar
type: Person
given name: Sahil
family name: Kumar

HTML Summary of #3184 Enhancing Immunogenicity of a Thermostable, Efficacious SARS-CoV-2 Vaccine Formulation through Oligomerization and Adjuvant Choice
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primary topic: Enhancing Immunogenicity of a Thermostable, Efficacious SARS-CoV-2 Vaccine Formulation through Oligomerization and Adjuvant Choice

QR Microbiology
type: Concept