title: Nanoscale size impact of nanoparticle interaction and activity studies with urease
creator: Bhardwaj, Priya
creator: Bisht, Bhawana
creator: Bhalla, Vijayender
description: Herein, we provide insights into the size-dependent interactions of silver nanoparticles (AgNPs) with urease and their implications for enzyme inhibition. AgNPs with a size of 5 nm exhibited the strongest binding affinity of 66 nM, resulting in significant enzyme attachment, interfering enzyme conformation, and a consequent loss of activity. Mid-sized AgNPs, i.e., 20 and 50 nm, exhibited binding affinities of 712 and 616 nM, causing only slight structural alterations. In contrast, 100 nm AgNPs demonstrated a high binding affinity of 171 nM accompanied by a favorable enthalpic contribution and a pronounced inhibitory effect.
publisher: The Royal Society of Chemistry
date: 2025
type: Article
type: NonPeerReviewed
relation: http://dx.doi.org/10.1039/D4NR04417G
identifier:   Bhardwaj, Priya and Bisht, Bhawana and Bhalla, Vijayender  (2025) Nanoscale size impact of nanoparticle interaction and activity studies with urease.  Nanoscale, 17.  pp. 5000-5004.      
relation: http://crdd.osdd.net/open/3202/