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primary topic: Nanocatalysis of silver-nanobioprobe based supersensitive electrochemical detection of Salmonella serotypes targeting virulence protein

Nanocatalysis of silver-nanobioprobe based supersensitive electrochemical detection of Salmonella serotypes targeting virulence protein
type: AcademicArticle
type: Article
abstract: Herein, we report a supersensitive and specific detection of Salmonella employing nanocatalysis of silver nanoparticle (AgNp). A nanobioprobe was developed employing specific antibody (Ab) that binds to a peptide present in transmembrane protein of Salmonella. We have studied 7 surface-exposed peptide hits from conserved virulence proteins (PagC, ST50, PagN, CdtB and FliC). These peptides were experimentally evaluated by BLI (Bio layer interferometry) for their reactivity towards antisera raised against an admix of major Salmonella serogroups. The most promising peptide was used to generate Ab with binding affinity Kd of 5.6 Ã�Â 10â��9 M. The Ab exhibited high specificity towards entire Salmonella serotypes prevalent in foods, as illustrated by FACS (Fluorescence-activated cell sorting) study. The Ab-AgNp probe was blocked with a dual layer to prevent non-specific interactions, confirmed by employing BLI and TEM (Transmission electron microscopy). For the electrochemical detection, the autonanocatalysis of AgNp in presence of H2O2 was used to generate numerous Ag+Â resulting in an amplified signal that could detect 10 cells/mL. The relative standard deviation (RSD) was observed to be 4.5. The platform achieved recovery of 100â��112 calculated for 102 cells/mL. The performance was validated in milk, buffer peptone water (BPW) and tap water by spiking studies. The study highlights the effectiveness of efficiently blocked AgNp-mediated probes for the highly selective and sensitive detection of Salmonella, representing a significant advancement in bacterial sensing.
date: 2025
volume: 268
author list: authors
type: EPrint
type: ArticleEPrint
is part of: repository

Biosensors and Bioelectronics
type: Collection

Vijayender Bhalla
type: Person
given name: Vijayender
family name: Bhalla

Priya Bhardwaj
type: Person
given name: Priya
family name: Bhardwaj

Bhawana Bisht
type: Person
given name: Bhawana
family name: Bisht

Vedika
type: Person
family name: Vedika

Sakshi Chauhan
type: Person
given name: Sakshi
family name: Chauhan

Namita Basnal
type: Person
given name: Namita
family name: Basnal

Sagrika
type: Person
family name: Sagrika

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primary topic: Nanocatalysis of silver-nanobioprobe based supersensitive electrochemical detection of Salmonella serotypes targeting virulence protein