…
comment: The repository administrator has not yet configured an RDF license.
primary topic: The role of amino acid composition in the antibacterial activity of laterosporulin analogs

The role of amino acid composition in the antibacterial activity of laterosporulin analogs
type: AcademicArticle
type: Article
abstract: This study aimed to compare the antimicrobial activity of the structural analogs of a class IId bacteriocin, laterosporulin.The antimicrobial peptide laterosporulin3 (LS3), produced by a Brevibacillus sp. strain SKR3, showed 96% identity to earlier described laterosporulin (LS) and 60% to laterosporulin10 (LS10) in amino acid composition. LS3 has a molecular weight of 5613 Da, the same as LS, yet differs in amino acid composition. Notable differences were found in their antimicrobial activity. Crystal structures of LS3 and LS10 were compared with LS. The analysis revealed that an additional Lys residue in LS3 enhances its net positive charge, leading to increased antimicrobial activity. FITC labeling showed the role of surface-displayed cationic Lys residues in the activity.Crystal structures of three different laterosporulins isolated from various strains of Brevibacillus were compared to understand the structure/function relationship. Results obtained in this study suggest that subtle variations in the amino acid composition of laterosporulins, distribution, and surface accessibility of charged residues can profoundly affect their antimicrobial activity.
date: 2025
volume: 136
issue: 9
author list: authors
type: EPrint
type: ArticleEPrint
is part of: repository

Journal of Applied Microbiology
type: Collection

Vipul Solanki
type: Person
given name: Vipul
family name: Solanki

Suresh Korpole
type: Person
given name: Suresh
family name: Korpole

Krishan Gopal Thakur
type: Person
given name: Krishan Gopal
family name: Thakur

Piyush Baindara
type: Person
given name: Piyush
family name: Baindara

Shelley Sardul Singh
type: Person
given name: Shelley Sardul
family name: Singh

Pushpanjali Bhardwaj
type: Person
given name: Pushpanjali
family name: Bhardwaj

Vishakha Grover
type: Person
given name: Vishakha
family name: Grover

Nayudu Nallabelli
type: Person
given name: Nayudu
family name: Nallabelli

Sumeeta Kumari
type: Person
given name: Sumeeta
family name: Kumari

HTML Summary of #3204 The role of amino acid composition in the antibacterial activity of laterosporulin analogs
format: text/html
primary topic: The role of amino acid composition in the antibacterial activity of laterosporulin analogs