<> "The repository administrator has not yet configured an RDF license."^^ . <> . . . "Development of a thermostable and broadly neutralizing pan-sarbecovirus vaccine candidate"^^ . "Zoonotic spillover of sarbecoviruses to humans resulted in the SARS-CoV-1 outbreak in 2003 and the current COVID-19 pandemic caused by SARS-CoV-2. In both cases, the viral spike protein (S) is the principal target of neutralizing antibodies that prevent infection. Within the spike, the immunodominant receptor-binding domain (RBD) is the primary target of neutralizing antibodies in COVID-19 convalescent sera and vaccine recipients. We have constructed stabilized RBD derivatives of different sarbecoviruses: SARS-CoV-1 (Clade 1a), WIV-1 (Clade 1a), RaTG13 (Clade 1b), RmYN02 (Clade 2), and BtKY72 (Clade 3). Stabilization enhanced yield by 3-23-fold. The RBD derivatives were conformationally intact, as assayed by binding to multiple broadly neutralizing antibodies. The stabilized RBDs show significant enhancement in apparent Tm, exhibit resistance to a 2-h incubation at temperatures up to 60 °C in PBS in contrast to the corresponding WT RBDs, and show prolonged stability of over 15 days at 37 °C after lyophilization. In mice immunizations, both stabilization and trimerization significantly enhanced elicited neutralization titers by �100-fold. The stabilized RBD cocktail elicited highly neutralizing titers against both homologous and heterologous pseudoviruses. The immunogenicity of the vaccine formulation was assessed in both na\\\"\\ive and SARS-CoV-2 preimmunized mice, revealing an absence of immune imprinting, thus indicating its suitability for use in future sarbecovirus-origin epidemics or pandemics."^^ . "2026-01" . "12" . "1" . . "American Chemical Society (ACS)"^^ . . . "ACS Infect. Dis."^^ . . . . . . . . . . . . . . . . . . . . . . . . . . . . . "Rajesh Prakash"^^ . "Ringe"^^ . "Rajesh Prakash Ringe"^^ . . "Raju S"^^ . "Rajmani"^^ . "Raju S Rajmani"^^ . . "Sahil"^^ . "Kumar"^^ . "Sahil Kumar"^^ . . "Suman"^^ . "Mishra"^^ . "Suman Mishra"^^ . . "Somnath"^^ . "Dutta"^^ . "Somnath Dutta"^^ . . "Randhir"^^ . "Singh"^^ . "Randhir Singh"^^ . . "Simran"^^ . "Srivastava"^^ . "Simran Srivastava"^^ . . "Raghavan"^^ . "Varadarajan"^^ . "Raghavan Varadarajan"^^ . . . . . "HTML Summary of #3207 \n\nDevelopment of a thermostable and broadly neutralizing pan-sarbecovirus vaccine candidate\n\n" . "text/html" . . . "QR Microbiology"@en . .