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primary topic: Genomic insights into Marinospirillum alkalitolerans sp. nov., a novel PHB producing bacterium from an Indian impact crater, and an emended description of family Oceanospirillaceae

Genomic insights into Marinospirillum alkalitolerans sp. nov., a novel PHB producing bacterium from an Indian impact crater, and an emended description of family Oceanospirillaceae
type: AcademicArticle
type: Article
abstract: Two Gram-stain-negative bacterial strains (MEB164T and MEB148), were isolated from haloalkaline waters of Lonar, crater lake, India. The strains were helical, non-spore-forming and motile with polar tufts of flagella. Optimal growth occurred at 37 â��, at pH 10 and with 3% (w/v) NaCl. 16S rRNA gene sequences of strains (MEB164T and MEB148) demonstrated the highest similarity with Marinospirillum alkaliphilum DSM 21637T (97.8%) followed by Marinospirillum celere DSM 18438T (96%). The genome size of strain MEB164T was determined to be 2.8 Mb with genomic DNA G + C contents of 53.1 mol %. ANI and dDDH values between strain MEB164T and the most closely related type strain M. alkaliphilum DSM 21637T were (72.3% and 21.4 ± 2.3%) while (AAI and POCP) values were (66.8 and 70.8%), respectively. Strain MEB164T exhibited potential for polyhydroxybutyrate (PHB) production, supported by key genes involved in PHB metabolism. Genome analysis further revealed presence of various pH tolerance genes, highlighting its adaptation to Lonar Lake. The predominant cellular fatty acids were summed feature 8 (C18:1 ω7c/C18:1 ω6c), C16:0 and summed feature 3 (C16:1 ω7c/C16:1 ω6c). The major respiratory quinone was ubiquinone-8. The prevalent polar lipids were diaminophosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, two unidentified amino phospholipids, three unidentified phospholipids and one unidentified lipid. Polyphasic analysis suggests that the two strains constitute a novel species within the genus Marinospirillum (family Oceanospirillaceae), proposed as Marinospirillum alkalitolerans sp. nov. The type strain is MEB164T (= JCM 35957T = MCC 5207T = NCIMB 15458T) and MEB148 (= JCM 35956 = MCC 5212 = NCIMB 15459) is an additional strain.
date: 2025-02
volume: 118
issue: 3
author list: authors
type: EPrint
type: ArticleEPrint
is part of: repository

Springer Science and Business Media LLC
type: Organization

Antonie Van Leeuwenhoek
type: Collection

Atharva Prabhu
type: Person
given name: Atharva
family name: Prabhu

Sonia Thite
type: Person
given name: Sonia
family name: Thite

Avinash Sharma
type: Person
given name: Avinash
family name: Sharma

Amit Yadav
type: Person
given name: Amit
family name: Yadav

Neetha Joseph
type: Person
given name: Neetha
family name: Joseph

Amaraja Joshi
type: Person
given name: Amaraja
family name: Joshi

Dibyajyoti Boruah
type: Person
given name: Dibyajyoti
family name: Boruah

Devika Godbole
type: Person
given name: Devika
family name: Godbole

Dev Kant Sindhu
type: Person
given name: Dev Kant
family name: Sindhu

Tushar Lodha
type: Person
given name: Tushar
family name: Lodha

http://crdd.osdd.net/open/3226/
title: HTML Summary of #3226 Genomic insights into Marinospirillum alkalitolerans sp. nov., a novel PHB producing bacterium from an Indian impact crater, and an emended description of family Oceanospirillaceae
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primary topic: Genomic insights into Marinospirillum alkalitolerans sp. nov., a novel PHB producing bacterium from an Indian impact crater, and an emended description of family Oceanospirillaceae