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primary topic: Differentiating α-synuclein aggregates using charge-sensitive gold nanoclusters

Differentiating α-synuclein aggregates using charge-sensitive gold nanoclusters
type: AcademicArticle
type: Article
abstract: The accumulation of α-synuclein (α-syn) aggregates in the brain is associated with Parkinson's disease, making it a promising biomarker for understanding the disease's pathogenesis. Distinguishing between oligomeric forms of amyloidogenic proteins is crucial, as their toxicity depends on conformation. This study leverages surface charge differences to distinguish α-syn monomers from amyloids. We employed label-free gold nanoclusters (AuNCs) with distinct surface charges to differentiate between wild-type and mutant A30P-$\alpha$-Syn based on their charge characteristics. Fluorescence spectroscopy and electrochemical measurements were performed to evaluate the sensitivity and interaction mechanisms. Our results show that Pro-AuNCs exhibit greater sensitivity towards monomeric forms of both wild-type and mutant $\alpha$-Syn, while His-AuNCs are more sensitive to amyloid forms. Impedance spectroscopy shows a detection limit in the picomolar range (pM), which is twice as sensitive as fluorescence measurements. Cell culture studies further validate our in vitro findings, demonstrating the effective specificity of AuNCs for detecting different conformations of $\alpha$-syn. Overall, our results indicate that this probe can successfully identify aggregated and monomeric forms of $\alpha$-syn, suggesting its potential for developing sensors aimed at understanding the etiology of Parkinson's disease.
date: 2025-08
volume: 17
issue: 32
author list: authors
type: EPrint
type: ArticleEPrint
is part of: repository

Royal Society of Chemistry (RSC)
type: Organization

Nanoscale
type: Collection

Sharmistha Sinha
type: Person
given name: Sharmistha
family name: Sinha

Harpreet Kaur
type: Person
given name: Harpreet
family name: Kaur

Deepak Sharma
type: Person
given name: Deepak
family name: Sharma

Arpit Tyagi
type: Person
given name: Arpit
family name: Tyagi

Ishani Sharma
type: Person
given name: Ishani
family name: Sharma

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primary topic: Differentiating α-synuclein aggregates using charge-sensitive gold nanoclusters