<didl:DIDL xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:didl="urn:mpeg:mpeg21:2002:02-DIDL-NS" xmlns:dii="urn:mpeg:mpeg21:2002:01-DII-NS" xmlns:dip="urn:mpeg:mpeg21:2002:01-DIP-NS" xmlns:dcterms="http://purl.org/dc/terms/" DIDLDocumentId="http://crdd.osdd.net/open/id/eprint/3232" xsi:schemaLocation="urn:mpeg:mpeg21:2002:02-DIDL-NS http://standards.iso.org/ittf/PubliclyAvailableStandards/MPEG-21_schema_files/did/didl.xsd urn:mpeg:mpeg21:2002:01-DII-NS http://standards.iso.org/ittf/PubliclyAvailableStandards/MPEG-21_schema_files/dii/dii.xsd urn:mpeg:mpeg21:2005:01-DIP-NS http://standards.iso.org/ittf/PubliclyAvailableStandards/MPEG-21_schema_files/dip/dip.xsd">
  <didl:Item>
    <didl:Descriptor>
      <didl:Statement mimeType="application/xml">
        <dii:Identifier>http://crdd.osdd.net/open/id/eprint/3232</dii:Identifier>
      </didl:Statement>
    </didl:Descriptor>
    <didl:Descriptor>
      <didl:Statement mimeType="application/xml">
        <dcterms:modified>2026-02-01T14:14:09Z</dcterms:modified>
      </didl:Statement>
    </didl:Descriptor>
    <didl:Component>
      <didl:Resource mimeType="application/xml" ref="http://crdd.osdd.net/open/cgi/export/eprint/3232/DIDL/open-eprint-3232.xml"/>
    </didl:Component>
    <didl:Item>
      <didl:Descriptor>
        <didl:Statement mimeType="application/xml">
          <dip:ObjectType>info:eu-repo/semantics/descriptiveMetadata</dip:ObjectType>
        </didl:Statement>
      </didl:Descriptor>
      <didl:Component>
        <didl:Resource mimeType="application/xml">
          <oai_dc:dc xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:dc="http://purl.org/dc/elements/1.1/" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
        <dc:title>Deciphering the underlying mechanism for Au/ZnO nanocomposites-induced modulation of structural features and thermodynamic stability of horse myoglobin</dc:title>
        <dc:creator>Kaur, Kiranjot</dc:creator>
        <dc:creator>Sharma, Deepak</dc:creator>
        <dc:creator>Haldar, Krishna Kanta</dc:creator>
        <dc:creator>Kumar, Rajesh</dc:creator>
        <dc:description>Au/ZnO nanocomposites (NCs) were synthesized and characterized by using various analytical techniques. Analysis of Au/ZnO NCs effect on 1H NMR, CD, fluorescence, and absorbance spectra of horse myoglobin (h-Mb) at 0.0 and 5.0 M urea (pH 7.4) revealed that the Au/ZnO NCs weaken the heme-globin interactions and also disrupt the secondary/tertiary structure of h-Mb. Furthermore, the Au/ZnO NCs effect of weakening the heme-globin interactions and disrupting the protein structures was detected more in the denaturant media than in the aqueous solution. Analysis of the Au/ZnO NCs effect on thermodynamic parameters (based on absorbance at 409 nm, CD at 222 nm, and DSC) of h-Mb at pH 7.4 revealed that the Au/ZnO NCs decrease the thermodynamic stability of h-Mb. Investigation of Au/ZnO NC's effects on urea concentration-dependent unfolding free energy of h-Mb at pH 7.4 showed that the Au/ZnO NCs strengthen the urea impact to decrease the thermodynamic stability of h-Mb. The quantitative estimation of enthalpic and entropic contributions to the Au/ZnO NCs-mediated decrease in the unfolding free energy of h-Mb reveals that the Au/ZnO NCs decrease the local (heme-globin interactions) and structural thermodynamic stability of the protein due to the enthalpic interactions of h-Mb with Au/ZnO NCs. ITC and time-resolved fluorescence studies of h-Mb further suggest that the Au/ZnO NCs form binding interactions with h-Mb at pH 7.4.</dc:description>
        <dc:publisher>American Chemical Society (ACS)</dc:publisher>
        <dc:date>2025-05</dc:date>
        <dc:type>Article</dc:type>
        <dc:type>NonPeerReviewed</dc:type>
        <dc:identifier>  Kaur, Kiranjot and Sharma, Deepak and Haldar, Krishna Kanta and Kumar, Rajesh  (2025) Deciphering the underlying mechanism for Au/ZnO nanocomposites-induced modulation of structural features and thermodynamic stability of horse myoglobin.  Langmuir, 41 (19).  pp. 12008-12021.      </dc:identifier>
        <dc:relation>http://crdd.osdd.net/open/3232/</dc:relation></oai_dc:dc>
        </didl:Resource>
      </didl:Component>
    </didl:Item>
    <didl:Item>
      <didl:Descriptor>
        <didl:Statement mimeType="application/xml">
          <dip:ObjectType>info:eu-repo/semantics/humanStartPage</dip:ObjectType>
        </didl:Statement>
      </didl:Descriptor>
      <didl:Component>
        <didl:Resource mimeType="application/html" ref="http://crdd.osdd.net/open/3232/"/>
      </didl:Component>
    </didl:Item>
  </didl:Item>
</didl:DIDL>