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primary topic: Overexpression of CBK1 or deletion of SSD1 confers fludioxonil resistance in yeast by suppressing Hog1 activation

Overexpression of CBK1 or deletion of SSD1 confers fludioxonil resistance in yeast by suppressing Hog1 activation
type: AcademicArticle
type: Article
abstract: Group III hybrid histidine kinases (HHK3) are known molecular targets of the widely used fungicidal agent fludioxonil which indirectly converts these kinases to a phosphatase form that causes constitutive activation of Hog1 MAPK. To better understand the fungicidal effect of fludioxonil we have screened S. cerevisiae haploid deletion collection for fludioxonil resistant mutant and identified Ssd1 as a critical factor for this. Deletion of SSD1 not only promoted resistance to fludioxonil but also abrogated Hog1 activation and other cellular damages caused by fludioxonil. Our results showed that fludioxonil perturbed the localization of Cbk1 kinase, an essential protein in yeast, at the bud neck triggering the accumulation of Ssd1 in P-bodies. As a result, localized synthesis of Ssd1 bound mRNA encoding cell wall proteins at the polarized growth site was impaired which created a sustained cell wall stress causing constitutive activation of Hog1. Our data, for the first time, clearly indicated the role of Cbk1 upstream of Hog1 and provided a novel paradigm in the mechanism of action of fludioxonil.
date: 2025-01
volume: 933
issue: 148905
author list: authors
type: EPrint
type: ArticleEPrint
is part of: repository

Elsevier BV
type: Organization

Gene
type: Collection

Rajendra Prasad
type: Person
given name: Rajendra
family name: Prasad

Anupam Sharma
type: Person
given name: Anupam
family name: Sharma

Alok K Mondal
type: Person
given name: Alok K
family name: Mondal

Soorya Partap Sasan
type: Person
given name: Soorya
family name: Partap Sasan

Mohd Wasi
type: Person
given name: Mohd
family name: Wasi

Yogita Martoliya
type: Person
given name: Yogita
family name: Martoliya

Debasree Kundu
type: Person
given name: Debasree
family name: Kundu

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primary topic: Overexpression of CBK1 or deletion of SSD1 confers fludioxonil resistance in yeast by suppressing Hog1 activation