creators_name: Kundu, Debasree
creators_name: Martoliya, Yogita
creators_name: Sharma, Anupam
creators_name: Partap Sasan, Soorya
creators_name: Wasi, Mohd
creators_name: Prasad, Rajendra
creators_name: Mondal, Alok K
type: article
datestamp: 2026-02-01 14:11:52
lastmod: 2026-02-01 14:11:52
metadata_visibility: show
title: Overexpression of CBK1 or deletion of SSD1 confers fludioxonil resistance in yeast by suppressing Hog1 activation
abstract: Group III hybrid histidine kinases (HHK3) are known molecular targets of the widely used fungicidal agent fludioxonil which indirectly converts these kinases to a phosphatase form that causes constitutive activation of Hog1 MAPK. To better understand the fungicidal effect of fludioxonil we have screened S. cerevisiae haploid deletion collection for fludioxonil resistant mutant and identified Ssd1 as a critical factor for this. Deletion of SSD1 not only promoted resistance to fludioxonil but also abrogated Hog1 activation and other cellular damages caused by fludioxonil. Our results showed that fludioxonil perturbed the localization of Cbk1 kinase, an essential protein in yeast, at the bud neck triggering the accumulation of Ssd1 in P-bodies. As a result, localized synthesis of Ssd1 bound mRNA encoding cell wall proteins at the polarized growth site was impaired which created a sustained cell wall stress causing constitutive activation of Hog1. Our data, for the first time, clearly indicated the role of Cbk1 upstream of Hog1 and provided a novel paradigm in the mechanism of action of fludioxonil.
date: 2025-01
publication: Gene
volume: 933
number: 148905
publisher: Elsevier BV
pagerange: 148905
citation:   Kundu, Debasree and Martoliya, Yogita and Sharma, Anupam and Partap Sasan, Soorya and Wasi, Mohd and Prasad, Rajendra and Mondal, Alok K  (2025) Overexpression of CBK1 or deletion of SSD1 confers fludioxonil resistance in yeast by suppressing Hog1 activation.  Gene, 933 (148905).  p. 148905.