TY  - JOUR
ID  - open3245
UR  - http://crdd.osdd.net/open/3245/
IS  - 118314
A1  - Modanwal, Radheshyam
A1  - Chaubey, Gaurav Kumar
A1  - Dilawari, Rahul
A1  - Talukdar, Sharmila
A1  - Sindhu, Anurag
A1  - Raje, Chaaya Iyengar
A1  - Raje, Manoj
Y1  - 2025/08//
N2  - Diabetes, especially type 2 diabetic mellitus (T2DM) is associated with increased risk of contracting tuberculosis (TB). Though several factors, including immune system dysfunction, have been linked to this sensitivity the exact reasons have yet to be fully elucidated. Although metformin, a diabetic medication, has been suggested as a potential supplementary for host-directed therapy of tuberculosis, the mechanisms of its anti-TB effects remain unclear. Extremely virulent pathogens including M. tb require copious supplies of iron for their survival within host. Macrophages are a key player of innate immunity, and a target for invading mycobacteria to colonize. Upon infection macrophages attempt to withhold this strategic mineral from the pathogen and clear it. We sought to ascertain as to how anti-hyperglycemic medications like metformin affect the regulation of iron metabolism and oxidative stress. Our findings demonstrate that metformin decreases intracellular iron levels by decreasing the endocytosis of iron carrier protein transferrin. Studying macrophages in a cell culture model (in-vitro and ex-vivo) as well as from an in-vivo rodent model we observed that the recruitment of both classical and non-classical transferrin receptors (CD71 and GAPDH) to their surface was decreased. Metformin was also found to induce mitochondrial ROS production though cellular ROS was inhibited. Since iron and mitochondrial reactive oxygen species (mitoROS) are essential for regulating intra cellular Mycobacterium tuberculosis growth, our current findings indicate that metformin could be the first choice in the treatment for T2DM2 in individuals from tuberculosis-endemic areas and also as an adjunct therapeutic for TB patients in general.
PB  - Elsevier BV
JF  - Biomed. Pharmacother.
VL  - 189
KW  - Iron; Metformin; Mycobacterium tuberculosis; ROS/mitoROS; Transferrin receptor; Tuberculosis
TI  - Metformin induces iron deprivation and enhances mitochondrial ROS in macrophages creating a hostile environment for survival of intracellular Mycobacterium tuberculosis
ER  -