creators_name: Modanwal, Radheshyam
creators_name: Chaubey, Gaurav Kumar
creators_name: Dilawari, Rahul
creators_name: Talukdar, Sharmila
creators_name: Sindhu, Anurag
creators_name: Raje, Chaaya Iyengar
creators_name: Raje, Manoj
type: article
datestamp: 2026-02-01 14:09:32
lastmod: 2026-02-01 14:09:32
metadata_visibility: show
title: Metformin induces iron deprivation and enhances mitochondrial ROS in macrophages creating a hostile environment for survival of intracellular Mycobacterium tuberculosis
keywords: Iron; Metformin; Mycobacterium tuberculosis; ROS/mitoROS; Transferrin receptor; Tuberculosis
abstract: Diabetes, especially type 2 diabetic mellitus (T2DM) is associated with increased risk of contracting tuberculosis (TB). Though several factors, including immune system dysfunction, have been linked to this sensitivity the exact reasons have yet to be fully elucidated. Although metformin, a diabetic medication, has been suggested as a potential supplementary for host-directed therapy of tuberculosis, the mechanisms of its anti-TB effects remain unclear. Extremely virulent pathogens including M. tb require copious supplies of iron for their survival within host. Macrophages are a key player of innate immunity, and a target for invading mycobacteria to colonize. Upon infection macrophages attempt to withhold this strategic mineral from the pathogen and clear it. We sought to ascertain as to how anti-hyperglycemic medications like metformin affect the regulation of iron metabolism and oxidative stress. Our findings demonstrate that metformin decreases intracellular iron levels by decreasing the endocytosis of iron carrier protein transferrin. Studying macrophages in a cell culture model (in-vitro and ex-vivo) as well as from an in-vivo rodent model we observed that the recruitment of both classical and non-classical transferrin receptors (CD71 and GAPDH) to their surface was decreased. Metformin was also found to induce mitochondrial ROS production though cellular ROS was inhibited. Since iron and mitochondrial reactive oxygen species (mitoROS) are essential for regulating intra cellular Mycobacterium tuberculosis growth, our current findings indicate that metformin could be the first choice in the treatment for T2DM2 in individuals from tuberculosis-endemic areas and also as an adjunct therapeutic for TB patients in general.
date: 2025-08
publication: Biomed. Pharmacother.
volume: 189
number: 118314
publisher: Elsevier BV
pagerange: 118314
citation:   Modanwal, Radheshyam and Chaubey, Gaurav Kumar and Dilawari, Rahul and Talukdar, Sharmila and Sindhu, Anurag and Raje, Chaaya Iyengar and Raje, Manoj  (2025) Metformin induces iron deprivation and enhances mitochondrial ROS in macrophages creating a hostile environment for survival of intracellular Mycobacterium tuberculosis.  Biomed. Pharmacother., 189 (118314).  p. 118314.