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primary topic: Characterization and genome analysis of a novel lytic phage vB_ntSalS-cftriSP11 infecting Salmonella typhimurium

Characterization and genome analysis of a novel lytic phage vB_ntSalS-cftriSP11 infecting Salmonella typhimurium
type: AcademicArticle
type: Article
abstract: BACKGROUND: In response to rising antibiotic resistance, bacteriophage application is gaining renewed attention, marking a paradigm shift in managing bacterial infections, especially foodborne pathogens. Non-typhoidal salmonellosis remains a major global health burden. In this context, a novel lytic bacteriophage targeting Salmonella typhimurium was isolated and characterized. METHODS AND RESULTS: A novel phage, vB_ntSalS-cftriSP11, targeting S. typhimurium, was isolated using spot and double agar overlay methods. One-step growth analysis showed a burst size of 146 ± 10 particles with a 20 min latency period. The phage exhibited a broad host range and stability across a wide pH and temperature range. Morphological and genomic analyses identified it as a member of Casjensviridae, with a 56,837 bp genome encoding 103 of 105 predicted ORFs linked to biological processes, cellular components, and molecular functions. Notably, no toxin genes, virulence factors, or antimicrobial resistance genes were detected in the genome. BLAST and phylogenetic analyses revealed high sequence similarity (97-98%) with known Salmonella phages 35, 37 and PK4. Functionally, the phage demonstrated strong anti-Salmonella activity, reducing biofilm biomass by 1.86-fold and 2.11-fold in 48- and 72-h-old biofilms, respectively, after 24 h of treatment. It also significantly decreased Salmonella counts on chicken breast samples at RT and 4 â�� at MOI 1 compared to the culture control. CONCLUSIONS: The Phage vB_ntSalS-cftriSP11 was identified as a stable, lytic bacteriophage with a broad host range and strong antibiofilm activity against S typhimurium. Its genetic safety profile and efficacy highlight its potential as a promising biocontrol agent against antibiotic-resistant Salmonella infections.
date: 2025-08
volume: 52
issue: 1
author list: authors
type: EPrint
type: ArticleEPrint
is part of: repository

Springer Science and Business Media LLC
type: Organization

Mol. Biol. Rep.
type: Collection

Bhawana Bisht
type: Person
given name: Bhawana
family name: Bisht

Nalina Murthy
type: Person
given name: Nalina
family name: Murthy

Priya Bhardwaj
type: Person
given name: Priya
family name: Bhardwaj

Poornima Priyadarshini
type: Person
given name: Poornima
family name: Priyadarshini

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primary topic: Characterization and genome analysis of a novel lytic phage vB_ntSalS-cftriSP11 infecting Salmonella typhimurium