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primary topic: http://crdd.osdd.net/open/id/eprint/3249

http://crdd.osdd.net/open/id/eprint/3249
type: AcademicArticle
type: Article
title: Cellulophaga algicola alginate lyase and Pseudomonas aeruginosa Psl glycoside hydrolase inhibit biofilm formation by Pseudomonas aeruginosa CF2843 on three-dimensional aggregates of lung epithelial cells
abstract: Pseudomonas aeruginosa is an opportunistic pathogen that produces a biofilm containing the polysaccharides, alginate, Psl, and Pel, and causes chronic lung infection in cystic fibrosis patients. Others and we have previously explored the use of alginate lyases in inhibiting P. aeruginosa biofilm formation on plastic and lung epithelial cell monolayers. We now employ a more physiologically representative model system, i.e., three-dimensional aggregates of A549 lung epithelial cells cultured under conditions of microgravity in a rotary cell culture system to mimic the natural lung environment, and a previously isolated clinical strain, Pseudomonas aeruginosa CF2843 that we engineered by transposon-mediated integration to express Green Fluorescent Protein and for which we also report the complete genome sequence. Immunostaining and lectin binding studies indicated that the three-dimensional cell aggregates harbored sialylated and fucosylated epitopes as well as Muc1, Muc5Ac, and β-catenin on their surfaces, suggestive of mucin secretion and the presence of tight junctions, hallmark features of lung epithelial tissue. Using this validated model system with confocal microscopy and viable bacterial counts as readouts, we demonstrated that Cellulophaga algicola alginate lyase and Pseudomonas aeruginosa Psl glycoside hydrolase, but not Pseudomonas aeruginosa Pel glycoside hydrolase, inhibit biofilm formation by Pseudomonas aeruginosa on three-dimensional lung epithelial cell aggregates.
date: 2025-06
volume: 9
issue: 100265
author list: authors
type: EPrint
type: ArticleEPrint
is part of: repository

Elsevier BV
type: Organization

Biofilm
type: Collection

T N C Ramya
type: Person
given name: T N C
family name: Ramya

Srikrishna Subramanian
type: Person
given name: Srikrishna
family name: Subramanian

Shilpee Pal
type: Person
given name: Shilpee
family name: Pal

Neetu
type: Person
family name: Neetu

http://crdd.osdd.net/open/3249/
title: HTML Summary of #3249 Cellulophaga algicola alginate lyase and Pseudomonas aeruginosa Psl glycoside hydrolase inhibit biofilm formation by Pseudomonas aeruginosa CF2843 on three-dimensional aggregates of lung epithelial cells
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primary topic: http://crdd.osdd.net/open/id/eprint/3249