TY  - JOUR
ID  - open3266
UR  - http://crdd.osdd.net/open/3266/
IS  - 1
A1  - Sahu, Shivani
A1  - Baid, Navin
A1  - Aggarwal, Deepali
A1  - Sharma, Ankita
A1  - Gun, Manisha
A1  - Molla, Sahanawaz
A1  - Sinha, Anunay
A1  - Dwivedi, Ambey Prasad
A1  - Tuli, Amit
A1  - Sharma, Mahak
A1  - Khosla, Sanjeev
A1  - Sundaramurthy, Varadharajan
A1  - Kumar, Ashwani
Y1  - 2025/10//
N2  - Mycobacterium tuberculosis is a model intracellular pathogen. The spatial-localization of M. tuberculosis inside macrophages is poorly defined. Here, we determine the spatial-localization of M. tuberculosis inside macrophages with reference to the nucleus. Few M. tuberculosis cells are perinuclear, while most are peripheral. Perinuclear M. tuberculosis are transported to lysosomes, have low Adenosine Triphosphate/Adenosine Diphosphate, are non-replicating, and tolerate front-line anti-tubercular medicines. M. tuberculosis pathogenicity determines its spatial location. Virulent M. tuberculosis strains are peripheral. However, avirulent M. tuberculosis strains or attenuated deletion mutants are transported to lysosomes in the perinuclear area. Early Secreted Antigenic Target-6 and Culture Filtrate Protein-10 play a critical role in inhibiting mycobacterial transport to the perinuclear space. Induction of centripetal transport of pathogenic M. tuberculosis-laden cargoes to perinuclear region enhances M. tuberculosis's delivery to the lysosomes and reduces mycobacterial growth. Interferon-? directs M. tuberculosis to lysosomes by modulating their perinuclear localization. Interferon-? upregulates Transmembrane protein 55B and JNK-interacting protein 4 via transcription factor EB. Increased transmembrane protein 55B and JNK-interacting protein 4 levels tether M. tuberculosis-laden cargoes to the dynein motor, causing their perinuclear delivery to lysosomes. These findings shed light on how mycobacterial metabolism, reproduction, and drug susceptibility are connected to virulence-guided spatial localization.
PB  - Springer Science and Business Media LLC
JF  - Nat. Commun.
VL  - 16
TI  - Fundamental role of spatial positioning of Mycobacterium tuberculosis in mycobacterial survival in macrophages
ER  -