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primary topic: Genomic insights into novel extremotolerant bacteria isolated from the NASA Phoenix mission spacecraft assembly cleanrooms

Genomic insights into novel extremotolerant bacteria isolated from the NASA Phoenix mission spacecraft assembly cleanrooms
type: AcademicArticle
type: Article
abstract: BACKGROUND: Human-designed oligotrophic environments, such as cleanrooms, harbor unique microbial communities shaped by selective pressures like temperature, humidity, nutrient availability, cleaning reagents, and radiation. Maintaining the biological cleanliness of NASA's mission-associated cleanrooms, where spacecraft are assembled and tested, is critical for planetary protection. Even with stringent controls such as regulated airflow, temperature management, and rigorous cleaning, resilient microorganisms can persist in these environments, posing potential risks for space missions. RESULTS: During the Phoenix spacecraft mission, genomes of 215 bacterial isolates were sequenced and based on overall genome-related indices, 53 strains belonging to 26 novel species were recognized. Metagenome mapping indicated less than 0.1% of the reads associated with novel species, suggesting their rarity. Genes responsible for biofilm formation, such as BolA (COG0271) and CvpA (COG1286), were predominantly found in proteobacterial members but were absent in other non-spore-forming and spore-forming species. YqgA (COG1811) was detected in most spore-forming members but was absent in Paenibacillus and non-spore-forming species. Cell fate regulators, COG1774 (YaaT), COG3679 (YlbF, YheA/YmcA), and COG4550 (YmcA, YheA/YmcA), controlling sporulation, competence, and biofilm development processes, were observed in all spore-formers but were missing in non-spore-forming species. COG analyses further revealed resistance-conferring proteins in all spore-formers (n = 13 species) and eight actinobacterial species, responsible for enhanced membrane transport and signaling under radiation (COG3253), transcription regulation under radiation stress (COG1108), and DNA repair and stress responses (COG2318). Additional functional analysis revealed that Agrococcus phoenicis, Microbacterium canaveralium, and Microbacterium jpeli contained biosynthetic gene clusters (BGCs) for ε-poly-L-lysine, benefic…
date: 2025-05
volume: 13
issue: 1
author list: authors
type: EPrint
type: ArticleEPrint
is part of: repository

Springer Science and Business Media LLC
type: Organization

Microbiome
type: Collection

Anamika Rawat
type: Person
given name: Anamika
family name: Rawat

Júnia Schultz
type: Person
given name: Júnia
family name: Schultz

Pratyay Sengupta
type: Person
given name: Pratyay
family name: Sengupta

Srinivasan Krishnamurthi
type: Person
given name: Srinivasan
family name: Krishnamurthi

Niketan Patel
type: Person
given name: Niketan
family name: Patel

Karthik Raman
type: Person
given name: Karthik
family name: Raman

Nitin K Singh
type: Person
given name: Nitin K
family name: Singh

Tahira Jamil
type: Person
given name: Tahira
family name: Jamil

Shobhan Karthick Muthamilselvi Sivabalan
type: Person
given name: Shobhan Karthick Muthamilselvi
family name: Sivabalan

Alexandre Soares Rosado
type: Person
given name: Alexandre Soares
family name: Rosado

Intikhab Alam
type: Person
given name: Intikhab
family name: Alam

Kasthuri Venkateswaran
type: Person
given name: Kasthuri
family name: Venkateswaran

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primary topic: Genomic insights into novel extremotolerant bacteria isolated from the NASA Phoenix mission spacecraft assembly cleanrooms