@article{open3285,
          volume = {367},
          author = {Gunjan Vasudeva and Chandni Sidhu and Kalyan Vaid and Pragya Priyadarshini and Vanish Kumar and Muthu Krishnan and Balvinder Singh and Anil Kumar Pinnaka},
            note = {Copyright of this article belongs to Elsevier.},
           title = {Bioremediation of catecholic pollutants with novel oxygen-insensitive catechol 2,3-dioxygenase and its potential in biomonitoring of catechol in wastewater},
       publisher = {Elsevier Science},
         journal = {Environmental Pollution},
           pages = {125613},
            year = {2025},
        keywords = {Catechol 2,3-dioxygenase, Substrate-specificity, Molecular modeling, Biosensing, functional metagenomics},
             url = {http://crdd.osdd.net/open/3285/},
        abstract = {The oxygenases are essential in the bioremediation of xenobiotic pollutants. To overcome cultivability constraints, this study aims to identify new potential extradiol dioxygenases using the functional metagenomics approach. RW1-4CC, a novel catechol 2,3-dioxygenase, was isolated using functional metagenomics approach, expressed in a heterologous system, and characterized thoroughly using state-of-the-art techniques. The serial truncation mutations of the C-terminal tail increase the catalytic efficiency of truncated proteins against the 2,3-dihydroxybiphenyl (2,3-DHB). RW1-4CC lose its 50 of activity at 60{\^A} {\^A}?C, with its optimum temperature at 15{\^A} {\^A}?C, whereas the truncated proteins were found to be more stable at extended temperature range, i.e., both RW1-4CC-A and RW1-4CC-B retained 50 of their activity at 75{\^A} {\^A}?C, with their temperature optima at 55{\^A} {\^A}?C and 65{\^A} {\^A}?C, respectively. The molecular docking studies further confirmed the high binding affinity of truncated proteins for the 2,3-DHB than catechol. The molecular modeling analysis revealed the difference in iron-binding and substrate interacting environment of RW1-4CC and its truncated proteins. The efficiency of purified RW1-4CC to detect catechol was evaluated using a gold screen-printed electrode by cyclic voltammetry. RW1-4CC detected catechol in wastewater and artificial seawater up to the concentration of 100{\^A} {\^I}?m, which makes it reliable for catechol detection.}
}