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primary topic: Bioremediation of catecholic pollutants with novel oxygen-insensitive catechol 2,3-dioxygenase and its potential in biomonitoring of catechol in wastewater

Bioremediation of catecholic pollutants with novel oxygen-insensitive catechol 2,3-dioxygenase and its potential in biomonitoring of catechol in wastewater
type: AcademicArticle
type: Article
abstract: The oxygenases are essential in the bioremediation of xenobiotic pollutants. To overcome cultivability constraints, this study aims to identify new potential extradiol dioxygenases using the functional metagenomics approach. RW1-4CC, a novel catechol 2,3-dioxygenase, was isolated using functional metagenomics approach, expressed in a heterologous system, and characterized thoroughly using state-of-the-art techniques. The serial truncation mutations of the C-terminal tail increase the catalytic efficiency of truncated proteins against the 2,3-dihydroxybiphenyl (2,3-DHB). RW1-4CC lose its 50 of activity at 60 °C, with its optimum temperature at 15 °C, whereas the truncated proteins were found to be more stable at extended temperature range, i.e., both RW1-4CC-A and RW1-4CC-B retained 50 of their activity at 75 °C, with their temperature optima at 55 °C and 65 °C, respectively. The molecular docking studies further confirmed the high binding affinity of truncated proteins for the 2,3-DHB than catechol. The molecular modeling analysis revealed the difference in iron-binding and substrate interacting environment of RW1-4CC and its truncated proteins. The efficiency of purified RW1-4CC to detect catechol was evaluated using a gold screen-printed electrode by cyclic voltammetry. RW1-4CC detected catechol in wastewater and artificial seawater up to the concentration of 100 Î¼m, which makes it reliable for catechol detection.
date: 2025
volume: 367
status: peerReviewed
status: published
author list: authors
type: EPrint
type: ArticleEPrint
is part of: repository
subject: QR Microbiology

Elsevier Science
type: Organization

Environmental Pollution
type: Collection

Gunjan Vasudeva
type: Person
given name: Gunjan
family name: Vasudeva

Vanish Kumar
type: Person
given name: Vanish
family name: Kumar

Balvinder Singh
type: Person
given name: Balvinder
family name: Singh

Anil Kumar Pinnaka
type: Person
given name: Anil Kumar
family name: Pinnaka

Chandni Sidhu
type: Person
given name: Chandni
family name: Sidhu

Kalyan Vaid
type: Person
given name: Kalyan
family name: Vaid

Pragya Priyadarshini
type: Person
given name: Pragya
family name: Priyadarshini

Muthu Krishnan
type: Person
given name: Muthu
family name: Krishnan

HTML Summary of #3285 Bioremediation of catecholic pollutants with novel oxygen-insensitive catechol 2,3-dioxygenase and its potential in biomonitoring of catechol in wastewater
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primary topic: Bioremediation of catecholic pollutants with novel oxygen-insensitive catechol 2,3-dioxygenase and its potential in biomonitoring of catechol in wastewater

QR Microbiology
type: Concept