<> "The repository administrator has not yet configured an RDF license."^^ . <> . . . "Bioremediation of catecholic pollutants with novel oxygen-insensitive catechol 2,3-dioxygenase and its potential in biomonitoring of catechol in wastewater"^^ . "The oxygenases are essential in the bioremediation of xenobiotic pollutants. To overcome cultivability constraints, this study aims to identify new potential extradiol dioxygenases using the functional metagenomics approach. RW1-4CC, a novel catechol 2,3-dioxygenase, was isolated using functional metagenomics approach, expressed in a heterologous system, and characterized thoroughly using state-of-the-art techniques. The serial truncation mutations of the C-terminal tail increase the catalytic efficiency of truncated proteins against the 2,3-dihydroxybiphenyl (2,3-DHB). RW1-4CC lose its 50 of activity at 60 °C, with its optimum temperature at 15 °C, whereas the truncated proteins were found to be more stable at extended temperature range, i.e., both RW1-4CC-A and RW1-4CC-B retained 50 of their activity at 75 °C, with their temperature optima at 55 °C and 65 °C, respectively. The molecular docking studies further confirmed the high binding affinity of truncated proteins for the 2,3-DHB than catechol. The molecular modeling analysis revealed the difference in iron-binding and substrate interacting environment of RW1-4CC and its truncated proteins. The efficiency of purified RW1-4CC to detect catechol was evaluated using a gold screen-printed electrode by cyclic voltammetry. RW1-4CC detected catechol in wastewater and artificial seawater up to the concentration of 100 μm, which makes it reliable for catechol detection."^^ . "2025" . "367" . . "Elsevier Science"^^ . . . "Environmental Pollution"^^ . . . . . . . . . . . . . . . . . . . . . . . . . . . . . "Gunjan"^^ . "Vasudeva"^^ . "Gunjan Vasudeva"^^ . . "Vanish"^^ . "Kumar"^^ . "Vanish Kumar"^^ . . "Balvinder"^^ . "Singh"^^ . "Balvinder Singh"^^ . . "Anil Kumar"^^ . "Pinnaka"^^ . "Anil Kumar Pinnaka"^^ . . "Chandni"^^ . "Sidhu"^^ . "Chandni Sidhu"^^ . . "Kalyan"^^ . "Vaid"^^ . "Kalyan Vaid"^^ . . "Pragya"^^ . "Priyadarshini"^^ . "Pragya Priyadarshini"^^ . . "Muthu"^^ . "Krishnan"^^ . "Muthu Krishnan"^^ . . . . . "HTML Summary of #3285 \n\nBioremediation of catecholic pollutants with novel oxygen-insensitive catechol 2,3-dioxygenase and its potential in biomonitoring of catechol in wastewater\n\n" . "text/html" . . . "QR Microbiology"@en . .