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primary topic: Determining factors for spermidine-induced modulation of conformational stability and dynamics of horse myoglobin

Determining factors for spermidine-induced modulation of conformational stability and dynamics of horse myoglobin
type: AcademicArticle
type: Article
abstract: The present work describes how water-miscible natural polyamine (NPA) spermidine (SPD) alters the conformational stability and dynamics of horse myoglobin (h-Mb) at pH 7.4. Analysis of thermal- and chemical-induced unfolding profiles of h-Mb at different concentrations of SPD at pH 7.4 revealed that SPD (\geq5 mM) reduces the conformational stability of h-Mb. The effect of SPD was further corroborated using MD simulations, which showed that SPD (\geq5 mM) enhanced the conformational fluctuations and reduced the structural stability of h-Mb. The SPD concentration effect on the thermodynamic equilibria (KU) of h-Mb was translated to the changes in preferential interaction coefficient (ΔΓ23) and hydration number (ΔΓw). The finding of the positive value of ΔΓ23 and the negative value of ΔΓw suggests that the interaction of SPD with h-Mb and the exclusion of water from the protein reduce the conformational stability of h-Mb. Analysis of SPD effects on enthalpy-entropy plots and temperature dependence of unfolding free energy of h-Mb suggests that the SPD forms soft, attractive enthalpic interactions with h-Mb. Analysis of SPD impact on the urea-concentration-dependent thermal unfolding midpoint temperature showed that SPD exhibits an additive effect to the urea-mediated decrease in the thermal stability of h-Mb.
date: 2025-07
volume: 64
issue: 13
status: peerReviewed
status: published
author list: authors
type: EPrint
type: ArticleEPrint
is part of: repository
subject: QR Microbiology

American Chemical Society (ACS)
type: Organization

Biochemistry
type: Collection

Rajesh Kumar
type: Person
given name: Rajesh
family name: Kumar

Deepak Sharma
type: Person
given name: Deepak
family name: Sharma

Manisha Yadav
type: Person
given name: Manisha
family name: Yadav

Sureshan Muthusamy
type: Person
given name: Sureshan
family name: Muthusamy

Saraboji Kadhirvel
type: Person
given name: Saraboji
family name: Kadhirvel

HTML Summary of #3289 Determining factors for spermidine-induced modulation of conformational stability and dynamics of horse myoglobin
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primary topic: Determining factors for spermidine-induced modulation of conformational stability and dynamics of horse myoglobin

QR Microbiology
type: Concept